Regulated expression of the Escherichia coli dam gene.

J Bacteriol

Department of Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, Massachusetts 01655, USA.

Published: August 2003

Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC166483PMC
http://dx.doi.org/10.1128/JB.185.16.5012-5014.2003DOI Listing

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