AI Article Synopsis
- The study examines how beta-estradiol affects the expression of the NaPi-IIb cotransporter, which is responsible for sodium-dependent phosphate absorption in the intestines.
- Estrogen treatment resulted in a significant increase in both the absorption of phosphate and the expression of NaPi-IIb mRNA and protein in rat intestines and human Caco-2 cells.
- The findings suggest that estrogen enhances intestinal phosphate absorption by activating the gene transcription of the NaPi-IIb transporter.
Article Abstract
The current experiments were designed to study the effect of beta-estradiol on type IIb sodium-coupled phosphate (NaPi-IIb) cotransporter gene expression. Uptake studies with intestinal brush-border membrane vesicles (BBMV) showed that estrogen treatment increased sodium-dependent phosphate absorption by approximately 45% in rat intestine. Northern blot analysis indicated that NaPi-IIb mRNA expression was increased by approximately 50% after estrogen treatment. Western blot analysis also detected an increase in BBMV NaPi-IIb protein expression in estrogen-treated rats. In human intestinal Caco-2 cells, NaPi-IIb mRNA abundance was increased approximately 60% after estrogen treatment, and this increase could be abolished by inhibition of gene transcription. Transfection studies with human NaPi-IIb promoter reporter constructs showed that the promoter was responsive to estrogen treatment. These studies demonstrate for the first time that estrogen stimulates intestinal sodium-dependent phosphate absorption in female rats. This stimulation is associated with increased NaPi-IIb mRNA and protein expression. Thus the effect of estrogen on intestinal Pi absorption may be partially due to activation of NaPi-IIb gene transcription.
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| http://dx.doi.org/10.1152/ajpgi.00172.2003 | DOI Listing |
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