Methylene blue is a basic dye commonly used in histologic microbiologic, and tissue staining. This report describes an instance of methylene blue toxicity in a premature neonate.
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Dumbbell probe-bridged CRISPR/Cas13a and nicking-mediated DNA cascade reaction for highly sensitive detection of colorectal cancer-related microRNAs.
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Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Sichuan Province Engineering Technology Research Center of Molecular Diagnosis of Clinical Diseases, Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, Sichuan, 646000, China. Electronic address:
Colorectal cancer (CRC) is a leading cause of cancer-related deaths globally, necessitating the development of sensitive and minimally invasive diagnostic approaches. In this study, we present a novel diagnostic strategy by integrating dumbbell probe-mediated CRISPR/Cas13a with nicking-induced DNA cascade reaction (DP-bridged Cas13a/NDCR) for highly sensitive microRNA (miRNA) detection. Target miRNA triggers Cas13a-mediated cleavage of the dumbbell probe, releasing an intermediate strand that hybridizes with a methylene blue-labeled hairpin probe on the electrode surface.
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J Mol Model
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Processes, Materials and Environment Laboratory (LPME), Faculty of Sciences and Technology of Fez, Sidi Mohamed Ben Abdellah University, B.P. 2202, Fez, Morocco.
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Department of Mathematical and Physical Sciences, Catholic University of Temuco, Temuco 4813302, Chile.
: A previous study investigated the in vitro release of methylene blue (MB), a widely used cationic dye in biomedical applications, from nanocellulose/nanoporous silicon (NC/nPSi) composites under conditions simulating body fluids. The results showed that MB release rates varied significantly with the nPSi concentration in the composite, highlighting its potential for controlled drug delivery. To further analyze the relationship between diffusion dynamics and the MB concentration, this study developed a finite element (FE) method to solve Fick's equations governing the drug delivery system.
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King Abdulaziz City for Science and Technology (KACST), Microelectronics and Semiconductors Institute, Mailbox 6086, Riyadh 11442, Saudi Arabia.
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Department of Mathematical, Physical and Computer Sciences, University of Parma, 43124 Parma, Italy.
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