Objective: To study morphology of different stage Blastocystis hominis (B. h) for establishing a base in the research of life cycle and pathogenicity of B. h and providing information for clinical laboratory.
Methods: B. h from diarrheal patients was continuously cultured in LES medium, and morphology of B. h was studied with iodine and iron hematoxylin staining under light microscope.
Results: The vacuolar, granular, amoeboid and cyst forms of B. h and transformation among the forms were observed microscopically.
Conclusion: Among different forms, the vacuolar and granular forms were often seen clinically and the vacuolar form can transform to cysts.
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