A rotavirus and a recombinant-enhanced green fluorescent protein from E. coli were concentrated 1.7 times and 1.5 times, respectively, by ultrafiltration at 37 degrees C and pH 7 using a pH-sensitive hydrogel, poly(N-isopropylacrylamide-co-N,N'-dimethylaminopropyl methacrylamide). Recoveries were 77% and 69%, respectively, and separation efficiencies were 58% and 44%, respectively. The concentration increase of the protein was confirmed by SDS-PAGE.
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| http://dx.doi.org/10.1023/a:1022312618548 | DOI Listing |
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