The homeobox gene PROX1 is related to the Drosophila prospero gene, which is expressed in the developing central nervous system and lens-secreting cone cells. We found that the PROX1 gene had missense and nonsense mutations in 4 of 29 hematologic cell lines analyzed. Decreased mRNA expression was also observed in half of these cell lines by RT-PCR. The restoration of PROX1 gene expression after treatment with the demethylating agent 5-aza-2'-deoxycytidine, as well as bisulfite sequencing analysis, indicated that gene silencing is caused by DNA hypermethylation at intron 1. Such hypermethylation was also seen in primary lymphomas (56.3%, 18/32) in a tumor-specific manner. These findings indicate that the profile of the PROX1 gene corresponds to that of a candidate tumor-suppressor gene.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/gcc.10248 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
In situ spatial transcriptomic analysis of human skeletal muscle using the Xenium platform.
Cell Tissue Res
January 2025
Department of Animal Science, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia.
Traditional transcriptomic studies often overlook the complex heterogeneity of skeletal muscle, as they typically isolate RNA from mixed muscle fibre and cell populations, resulting in an averaged transcriptomic profile that obscures fibre type-specific differences. This study assessed the potential of the recently developed Xenium platform for high-resolution spatial transcriptomic analysis of human skeletal muscle histological sections. Human vastus lateralis muscle samples from two individuals were analysed using the Xenium platform and Human Multi-Tissue and Cancer Panel targeting 377 genes complemented by staining of successive sections for Myosin Heavy Chain isoforms to differentiate between type 1 and type 2 muscle fibres.
View Article and Find Full Text PDFUpregulation of Differentiates Intrahepatic Cholangiocarcinoma from Hepatocellular Carcinoma and Both Colorectal and Pancreatic Adenocarcinoma Liver Metastases.
Genes (Basel)
November 2024
Institute of Pathology, Faculty of Medicine, University of Ljubljana, 1000 Ljubljana, Slovenia.
Background: Altered gene expression in cancers holds great potential to improve the diagnostics and differentiation of primary and metastatic liver cancers. In this study, the expression of the protein-coding genes ring finger protein 135 (), ephrin-B2 (), ring finger protein 125 (), homeobox-C 4 (), actin-binding LIM protein 1 () and oncostatin M receptor () and the long non-coding RNAs (lncRNA) prospero homeobox 1 antisense RNA 1 () and leukemia inhibitory factor receptor antisense RNA 1 () was investigated in hepatocellular carcinoma, cholangiocarcinoma, colorectal liver metastases and pancreatic ductal adenocarcinoma liver metastases.
Methods: This study included 149 formalin-fixed, paraffin-embedded samples from 80 patients.
Identification and Validation of Epithelial Cell Centre Regulatory Transcription Factors in the Gastric Cancer Microenvironment.
Int J Gen Med
December 2024
Department of Pathology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yun Nan, People's Republic of China.
Purpose: To identify the epithelial cell centre regulatory transcription factors in the gastric cancer (GC) microenvironment and provide a new strategy for the diagnosis and treatment of GC.
Methods: The GC single-cell dataset was downloaded from the Gene Expression Omnibus (GEO) database. The regulatory mechanisms of transcription factors in both pan-cancer and GC microenvironments were analysed using the Cancer Genome Atlas (TGCA) database.
The role of exercise in promoting lymphangiogenesis and extracellular matrix synthesis in lymphedema-induced tissue injury.
Mol Biol Rep
December 2024
Humanitas College, Kyung Hee University, 1732 Deogyeongdae-Ro, Yongin, 17104, South Korea.
Background: Secondary lymphedema is a progressive condition caused by lipid- and protein-rich interstitial fluid accumulation resulting from compromised lymphatic function. It commonly occurs in cancer patients following surgical lymph node ablation and radiation treatment. This study aims to elucidate the effects of exercise on the myokine interleukin (IL)-6 and the molecular changes involved in lymphangiogenesis and extracellular matrix (ECM) synthesis using a lymphedema mouse model.
View Article and Find Full Text PDFEngineering ADSCs by manipulating YAP for lymphedema treatment in a mouse tail model.
Exp Biol Med (Maywood)
December 2024
State Key Laboratory of Oral Diseases, National Center for Stomatology, National Clinical Research Center for Oral Diseases, Department of Oral and Maxillofacial Surgery, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, China.
Article Synopsis
- Secondary lymphedema is a chronic condition causing limb deformities and dysfunction, and while traditional treatments exist, they are not curative.
- This study explores the use of engineered adipose-derived stem cells (ADSCs) for treating lymphedema, specifically focusing on the role of a protein called Yes-associated protein (YAP).
- Results showed that manipulating YAP expression in ADSCs enhanced their effectiveness, leading to improved lymphatic vessel formation and reduced fibrosis, suggesting a potential new therapeutic approach for lymphedema.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!