Statement Of Problem: There are many bonding systems available, with or without a prior acid etch. The mechanisms differ from system to system. Therefore it is important that the clinician know which are the most effective and which provide the best seal.
Purpose: This study measured the infiltration of physiological saline solution across dentin after application of self-etching or 1-bottle dentin bonding systems.
Materials And Methods: Thirty-six extracted noncarious human third molars from patients 18 to 25 years old were used for this study. Dentin disks were cut from crown segments parallel to the occlusal surface at the incisal portion of the pulp cavity. The 36 disks, each 1 mm thick, were divided into 6 groups (n=6 per group), each of which received one of the following dentin bonding systems: Optibond Solo Plus and Excite, which have ethanol as the solvent; Prime & Bond NT, which has acetone as the solvent; Single Bond, which has a mixed solvent; and Clearfil SE Bond or Prompt L-Pop, both self-etching systems The hydraulic conductance, the volume of fluid transported across a known area of surface (0.28 cm(2)) per unit time under a unit pressure gradient (200 cm H(2)O), was analyzed for the self-etching and 1-bottle dentin bonding systems with the Flodec apparatus. Both sides of each specimen were etched with 36% phosphoric acid for 30 seconds. Measurements were made every 30 seconds for 15 minutes. The initial measurement served as the reference value for each specimen. The measurements were repeated when a smear layer had been formed and, finally, after one of the 6 bonding systems had been applied. The data were analyzed by use of analysis of variance and a posteriori tests (Fischer's PLSD, Duncan's new multiple range) with alpha=.05.
Results: The greatest mean reduction (40%, P<.05) in penetration was observed with the 4 products with conventional etching: Optibond Solo Plus, Single Bond, Excite, and Prime & Bond NT. The self-etching products were associated with reductions of 36% (Clearfil SE Bond) and 16% (Prompt L Pop). Standard deviations were high, 50% of the mean value, except for the Single Bond group (-42 +/- 13) and Prime & Bond NT (-41 +/- 14).
Conclusion: Within the limitations of this study, the 4 bonding systems tested with a phosphoric acid before etching provided a greater decrease in dentin permeability than the 2 self-etching systems. The difference in permeability decrease was significant for Prompt L-Pop but not for Clearfil SE Bond.
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http://dx.doi.org/10.1016/s0022-3913(03)00258-0 | DOI Listing |
Nat Chem
January 2025
SAMS Research Group, Université de Strasbourg, CNRS, Institut Charles Sadron UPR 22, Strasbourg, France.
Molecular spin qubits have the advantages of synthetic flexibility and amenability to be tailored to specific applications. Among them, chromophore-radical systems have emerged as appealing qubit candidates. These systems can be initiated by light to form triplet-radical pairs that can result in the formation of quartet states by spin mixing.
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January 2025
Department of Chemistry, Queen's University, Kingston, ON K7L 3N6, Canada.
Tambjamines are complex bipyrrole-containing natural products that possess promising bioactive properties. Although is known to produce both cyclic tambjamine MYP1 and the linear precursor (YP1), the biosynthetic machinery used to catalyze the site-selective oxidative carbocyclization at the unactivated 1° carbon of YP1 has remained unclear. Here, we demonstrate that a three-component Rieske system consisting of an oxygenase (TamC) and two redox partner proteins is responsible for this unprecedented activity on YP1 and potentially, a non-native substrate (BE-18591).
View Article and Find Full Text PDFSLAS Discov
January 2025
Bonds Biosystems, 27 Strathmore Rd, Natick, MA, USA. Electronic address:
Obesity and type 2 diabetes (T2D) are strongly linked to abnormal adipocyte metabolism and adipose tissue (AT) dysfunction. However, existing adipose tissue models have limitations, particularly in the stable culture of fat cells that maintain physiologically relevant phenotypes, hindering a deeper understanding of adipocyte biology and the molecular mechanisms behind differentiation. Current model systems fail to fully replicate in vivo metabolism, posing challenges in adipose research.
View Article and Find Full Text PDFBiosens Bioelectron
January 2025
Key Lab for Special Functional Materials of Ministry of Education, and School of Nanoscience and Materials Engineering, Henan University, 475004, Kaifeng, China. Electronic address:
Influenza A virus (IAV) and influenza B virus (IBV) with similar symptoms of infection caused a serious disease burden and economic losses in annual epidemic season, so it is important to quickly and accurately detect and distinguish between IAV and IBV during influenza season. Herein, the quantum dot microspheres (QDMS) were synthesized and applied to lateral flow immunoassays (LFIA), and a point-of-care (POC) biosensor that can discriminately and simultaneously diagnose IAV and IBV within 10 min was established. A double-sandwich QDMS nanotags was synthesized by immobilizing hydrophobic quantum dots (QDs) with chemical bonding method on a silica sphere template with an outer silica shell protection showed excellent stability and high fluorescence.
View Article and Find Full Text PDFJ Phys Chem B
January 2025
Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104, United States.
Computationally designed 29-residue peptides yield tetra-α-helical bundles with symmetry. The "bundlemers" can be bifunctionally linked via thiol-maleimide cross-links at their N-termini, yielding supramolecular polymers with unusually large, micrometer-scale persistence lengths. To provide a molecularly resolved understanding of these systems, all-atom molecular modeling and simulations of linked bundlemers in explicit solvent are presented.
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