We collected and completely sequenced 28,469 full-length complementary DNA clones from Oryza sativa L. ssp. japonica cv. Nipponbare. Through homology searches of publicly available sequence data, we assigned tentative protein functions to 21,596 clones (75.86%). Mapping of the cDNA clones to genomic DNA revealed that there are 19,000 to 20,500 transcription units in the rice genome. Protein informatics analysis against the InterPro database revealed the existence of proteins presented in rice but not in Arabidopsis. Sixty-four percent of our cDNAs are homologous to Arabidopsis proteins.
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http://dx.doi.org/10.1126/science.1081288 | DOI Listing |
Curr Issues Mol Biol
December 2024
Department of Fisheries Science, Chonnam National University, Yeosu 59626, Republic of Korea.
Myogenic regulator factors (MRFs) are essential for skeletal muscle development in vertebrates, including fish. This study aimed to characterize the role of () in muscle development in Nile tilapia by cloning from muscle tissues. To explore the function of , CRISPR/Cas9 gene editing was employed.
View Article and Find Full Text PDFPlant Dis
December 2024
Honghe University, College of Biological and Agricultural Sciences, Mengzi, Yunnan, China;
The Asteraceae family plant Erigeron breviscapus (Vant.) Hand.-Mazz.
View Article and Find Full Text PDFGenes Cells
January 2025
Department of Anatomy and Cell Biology, Research Institute of Pharmaceutical Science, Faculty of Pharmacy, Musashino University, Tokyo, Japan.
We previously suggested that the signal transducer and activator of transcription 1 (STAT1) gene is autoregulated in an interferon (IFN)-dependent manner via a distal regulatory region approximately 5.5-6.2 kb upstream of the murine and human STAT1 promoters (designated 5.
View Article and Find Full Text PDFPlant Cell Rep
December 2024
Institute of Nanfan & Seed Industry, Guangdong Academy of Sciences, Guangzhou, 510000, Guangdong, China.
A total of 24 genes of vacuolar H-translocating pyrophosphatases H-PPases (VPP) genes were identified in Saccharum spontaneum AP85-441 and the ScVPP1-overexpressed Arabidopsis plants conferred salt tolerance. The vital role of vacuolar H-translocating pyrophosphatases H-PPases (VPP) genes involved in plants in response to abiotic stresses. However, the understanding of VPP functions in sugarcane remained unclear.
View Article and Find Full Text PDFComp Biochem Physiol C Toxicol Pharmacol
December 2024
Key Laboratory of Marine Ecological Conservation and Restoration, Third Institute of Oceanography, Ministry of Natural Resources, Xiamen, China. Electronic address:
Cathelicidin is a family of antimicrobial peptides in vertebrates that plays an important role in resistance and immunization against pathogenic microorganisms. In the present study, the full-length cDNA sequences of four novel cathelicidins (cathelicidin-1 to cathelicidin-4) in the tiger frog Hoplobatrachus rugulosus, encoding 153, 188, 132, and 160 amino acids, respectively, were firstly cloned by rapid amplification of the cDNA ends (RACE) technique. Sequence comparison and phylogenetic tree analysis indicated that the structures of the four cathelicidins are highly diverse.
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