Objective: To investigate the chromatin status of oocytes and their ploidity in order to consider possible genetic reasons for their unsuccessful fertilization in vitro.
Study Design: Eighty-six unfertilized in vitro human oocytes were scored in six groups according to their chromatin status. Analysis was performed by cytogenetics and by fluorescent in situ hybridization (FISH).
Results: 73.26% of oocytes were successfully analyzed: 44.19% by classical cytogenetics and 17.44% by FISH. 11.63% of the analyzed cells contained sperm chromosomes with premature chromosome condensation (PCC). Among karyotyped oocytes, a high aneuploidy rate was found (5.26% haploid abnormal, 21.05% hypohaploid, 36.84% hyperhaploid and 7.89% diploid). FISH for chromosomes X, 1, 5 and 19 performed on unsuitable for karyotyping metaphase plates revealed 13.33% aneuploid cells.
Conclusion: Analysis of fertilization failure could be more informative when a combination of methods is applied.
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