AI Article Synopsis
- The study aims to explore how the NGF family and their receptors are expressed in gastric carcinoma compared to normal gastric tissue and to understand their impact on cancer development.
- The methods involved isolating RNA from gastric cancer and normal gastric tissues, conducting hybridization on Atlas Array membranes, and analyzing the differential expression of mRNA through specialized software.
- Results showed that while both NGF family and Trk family receptors were found in both tissue types, their expression was notably down-regulated in gastric carcinoma, particularly NT-3 and BDGF, suggesting they may play a critical role in cancer cell death and the progression of gastric cancer.
Article Abstract
Aim: To investigate the expression of NGF family and their receptors in gastric carcinoma and normal gastric mucosa, and to elucidate their effects on gastric carcinoma.
Methods: RNA of gastric cancer tissues and normal gastric tissues was respectively isolated and mRNA was purified. Probes of both mRNA reverse transcription product cDNAs labeled with alpha-(33)P dATP were respectively hybridized with Atlas Array membrane where NGF and their family genes were spotted on. Hybridized signal images were scanned on phosphor screen with ImageQuant 5.1 software after hybridization. Normalized values on spots were analyzed with ArrayVersion 5.0 software. Differential expression of NGF family and their receptors mRNA was confirmed between hybridized Atlas Array membranes of gastric cancer tissues and normal gastric mucosa, then their effects on gastric carcinoma were investigated.
Results: Hybridization signal images on Atlas Array membrane appeared in a lower level of nonspecific hybridization. Both of NGF family and their receptors Trk family mRNA were expressed in gastric cancer and normal gastric mucosa. But adversely up-regulated expression in other tissues and organs. NGF, BDGF, NT-3, NT-4/5, NT-6 and TrkA, B and C were down-regulated simultaneously in gastric carcinoma in comparison with normal gastric mucosa. Degrees of down-regulation in NGF family were greater than those in their receptors Trk family. Down-regulation of NT-3 and BDGF was the most significant, and TrkC down-regulation level was the lowest in receptors Trk family.
Conclusion: Down-regulated expression of NGF family and their receptors Trk family mRNA in gastric cancer is confirmed. NGF family and their receptors Trk family probably play a unique role in gastric cancer cell apoptosis by a novel Ras or Raf signal transduction pathway. Their synchronous effects are closely associated with occurrence and development of gastric carcinoma induced by reduction of signal transduction of programmed cell death.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4615477 | PMC |
| http://dx.doi.org/10.3748/wjg.v9.i7.1431 | DOI Listing |
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