A biosurfactant-producing strain, Bacillus licheniformis F2.2, was isolated from a fermented food in Thailand. The strain was capable of producing a new biosurfactant, BL1193, as well as two kinds of popular lipopeptide biosurfactants, plipastatin and surfactin. Mass spectrometry and FT-IR analysis indicated that BL1193 had a molecular mass of 1,193 Da with no peptide portion in the molecule. While plipastatin and surfactin were abundantly produced in a nutrient YPD medium, BL1193 was produced only in a synthetic DF medium containing no amino acids. According to an oil displacement activity test, the specific activity of BL1193 (6.53 kBS units/mg) is equivalent to that of surfactin (5.78-6.83 kBS units/mg).
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1271/bbb.67.1239 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Cell-to-Cell Natural Transformation Mediated Efficient Plasmid Transfer Between Species.
Int J Mol Sci
January 2025
State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
Horizontal gene transfer (HGT) plays a pivotal role in bacterial evolution, shaping the genetic diversity of bacterial populations. It can occur through mechanisms such as conjugation, transduction, and natural transformation. , a model Gram-positive bacterium, serves not only as a robust system for studying HGT but also as a versatile organism with established industrial applications, such as producing industrial enzymes, antibiotics, and essential metabolites.
View Article and Find Full Text PDFSalidroside production through cascade biocatalysis with a thermostability-enhanced UDP-glycosyltransferase.
Int J Biol Macromol
January 2025
School of Marine Sciences, Sun Yat-Sen University, Zhuhai 519080, China. Electronic address:
Salidroside is a phenylpropanoid glycoside with wide applications in the food, pharmaceutical, and cosmetic industries; however, the plant genus Rhodiola, the natural source of salidroside, has slow growth and limited distribution. In this study, we designed a novel six-enzyme biocatalytic cascade for the efficient production of salidroside, utilizing cost-effective bio-based L-Tyrosine as the starting material. A preliminary analysis revealed that the poor thermostability of the Bacillus licheniformis UDP-glycosyltransferase (EC 2.
View Article and Find Full Text PDFThe ionic liquid-assisted sample preparation method pTRUST allows sensitive proteome characterization of a variety of bacterial endospores to aid in the search for protein biomarkers.
PLoS One
January 2025
Department of Applied Chemistry, National Defense Academy, Kanagawa, Japan.
Bacterial endospores are ubiquitous and are responsible for various human infections. Recently, we reported that an ionic liquid (IL)-based sample preparation method (named pTRUST) facilitated highly efficient shotgun analysis of the Bacillus subtilis spore proteome in trace samples. In this study, we evaluated the efficiency and applicability of the pTRUST technology using three different spore preparations: one purified from the closely related subspecies B.
View Article and Find Full Text PDFHeterologous and High Production of Ergothioneine in by Using Genes from Anaerobic Bacteria.
Metabolites
January 2025
School of Biotechnology, Key Laboratory of Carbohydrate Chemistry, Biotechnology of Ministry of Education, Jiangnan University, Wuxi 214122, China.
Purpose: This study aimed to utilize genetically engineered for the production of ergothioneine (EGT). Given the value of EGT and the application of in enzyme preparation production, we cloned the key enzymes (EanA and EanB) from . Through gene alignment, new ergothioneine synthase genes (EanAN and EanBN) were identified and then expressed in to construct strains.
View Article and Find Full Text PDFSpecificity of Amino Acid Profiles Produced in Soybean Fermentations by Three spp.
J Microbiol Biotechnol
December 2024
Department of Food Science and Biotechnology, Kyonggi University, Suwon 16227, Republic of Korea.
We compared the salt tolerance and proteolytic activity of 120 strains of each of , , and . Most strains exhibited growth in 12% (w/v) NaCl and showed proteolytic activity in 10% or 11% NaCl. The majority of strains grew in 14% NaCl and showed proteolytic activity in 12% or 13% NaCl.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!