Evaluation of fungal burden in experimental paracoccidioidomycosis by using the fluorescent dye Blankophor.

J Clin Microbiol

Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, São Paulo, SP, Brazil.

Published: July 2003

The fungal load in organs and blood of susceptible and resistant mice infected with Paracoccidioides brasiliensis was quantitated by using the optical brightener Blankophor and compared with CFU counts. Fluorescent staining of fungal cells proved to be a quick and easy procedure, suitable for evaluation of paracoccidioidomycotic infection.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC165288PMC
http://dx.doi.org/10.1128/JCM.41.7.3419-3422.2003DOI Listing

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