AI Article Synopsis
- The Na,K-ATPase ion pump's nucleotide-binding domain was expressed and purified from E. coli with a His tag, resulting in a 24 kDa protein.
- The protein was crystallized alongside NiCl(2), with the crystals falling under space group F23 and having specific unit-cell dimensions.
- Data was collected from both native and thimerosal-treated crystals frozen in sucrose, leading to the identification of five mercury positions and the calculation of initial SIR phases.
Article Abstract
The nucleotide-binding domain of the Na,K-ATPase ion pump was expressed with a His tag in Escherichia coli and purified. The soluble 24 kDa derivative consists of 214 amino-acid residues and was crystallized in the presence of NiCl(2). The crystals belong to space group F23, with unit-cell parameters a = b = c = 147.5 A, and diffract to 3.1 A. Complete data sets could be collected from native and thimerosal-treated crystals frozen in 50% sucrose. Five mercury positions were found and initial SIR phases calculated.
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| http://dx.doi.org/10.1107/s0907444903008795 | DOI Listing |
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