AI Article Synopsis
- The study investigates how dendritic cells (DCs) interact with the intracellular pathogen Legionella pneumophila, showing that DCs can limit its growth, unlike other immune cells.
- The research found that after DCs engulf Legionella, its phagosomes transform into organelles derived from the endoplasmic reticulum (ER), but this doesn't prevent Legionella antigens from being presented on MHC class II molecules.
- Ultimately, the study highlights the role of DCs in presenting Legionella proteins to T cells, suggesting that their ability to control Legionella growth could be key to triggering effective immune responses against such vacuolar pathogens.
Article Abstract
To understand how adaptive immune responses are generated against bacteria that avoid being delivered to lysosomes, interactions between professional antigen-presenting cells (APCs) and the intracellular pathogen Legionella pneumophila were examined. In contrast to murine bone marrow-derived macrophages (BMMs), we show that dendritic cells (DCs) restrict the growth of intracellular Legionella. Similar to what has been reported in BMMs, phagosomes containing Legionella matured into endoplasmic reticulum (ER)-derived organelles after DC internalization. Biogenesis of an ER-derived vacuole did not effectively sequester Legionella antigens from presentation on MHC class II molecules (MHC II). It was determined that proteins synthesized after Legionella had established residence in an ER-derived vacuole were presented by infected APCs. These data indicate that the ability of DCs to restrict intracellular growth of Legionella could be an important property that facilitates priming of protective T cell-mediated immune responses to vacuolar pathogens.
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| http://dx.doi.org/10.1016/s1074-7613(03)00140-7 | DOI Listing |
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