AI Article Synopsis
- In vitro glycorandomization (IVG) technology relies on quickly producing sugar phosphates, ideally using enzymatic synthesis instead of chemical methods.
- The study develops a high-throughput colorimetric assay for galactokinase (GalK) to investigate the enzyme's substrate specificity and kinetics in Escherichia coli.
- This new dinitrosalicylic assay is versatile and can potentially be used for various sugar-processing enzymes.
Article Abstract
In vitro glycorandomization (IVG) technology is dependent upon the ability to rapidly synthesize sugar phosphates. Compared with chemical synthesis, enzymatic (kinase) routes to sugar phosphates would be attractive for this application. This work focuses upon the development of a high-throughput colorimetric galactokinase (GalK) assay and its application toward probing the substrate specificity and kinetic parameters of Escherichia coli GalK. The demonstrated dinitrosalicylic assay should also be generally applicable to a variety of sugar-processing enzymes. [reaction: see text]
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| http://dx.doi.org/10.1021/ol034642d | DOI Listing |
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