We studied the behavior and differentiation of pluripotent embryonic stem cells of R1 mice in vivo. Undifferentiated embryonic stem cells and differentiating embryoid bodies grafted in abdominal cavity of the irradiated mice were shown to form tumors with derivatives of all germinal layers. Cells of the embryoid bodies form tumors two weeks after the grafting, while the undifferentiated embryonic stem cells form tumors only by week three.
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