A process for the reduction of residual solvents in spray-dried poly(lactide-co-glycolide) (PLGA)-darbepoetin alfa microparticles was developed using carbon dioxide (CO(2)) as an extraction solvent. CO(2) was investigated in two phase states, liquid and gas. Detrimental effects on encapsulated protein integrity and microparticle morphology were observed with liquid CO(2) exposure. Extraction with CO(2) gas at <100 psig reduced residual solvent concentration and particle agglomeration was limited. Extraction rates and particle agglomeration increased with higher CO(2) gas pressures. The CO(2) pressures below which particles of polylactide (PLA) and PLGA microparticles significantly agglomerated were determined and the data used to develop extraction cycles. Extraction cycles were developed in which CO(2) gas pressure was increased as residual solvent concentration decreased in order to keep extraction rates high throughout the cycle. Spray dried darbepoetin alfa-PLGA microparticles were extracted with CO(2) gas and characterized for residual solvent concentration, process yield, particle size distribution, morphology, and protein integrity. The results indicated CO(2) gas may be used to reduce residual solvent to approximately 200 ppm with no significant detrimental effects on protein integrity or microparticle morphology.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0168-3659(03)00152-4 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluating the effects of continuous veno-venous hemodiafiltration on O and CO removal and energy expenditure measurement using indirect calorimetry.
Ann Intensive Care
January 2025
Department of Intensive Care Medicine, Universitaire Ziekenhuis Brussel (UZ Brussel), Vrije Universiteit Brussel (VUB), Brussels, Belgium.
Background: Continuous veno-venous hemodiafiltration (CVVHDF) is used in critically ill patients, but its impact on O₂ and CO₂ removal, as well as the accuracy of resting energy expenditure (REE) measurement using indirect calorimetry (IC) remains unclear. This study aims to evaluate the effects of CVVHDF on O₂ and CO₂ removal and the accuracy of REE measurement using IC in patients undergoing continuous renal replacement therapy.
Design: Prospective, observational, single-center study.
Additives-Modified Electrodeposition for Synthesis of Hydrophobic Cu/CuO with Ag Single Atoms to Drive CO Electroreduction.
Adv Mater
January 2025
State Key Laboratory of Petroleum Molecular & Process Engineering, Shanghai Key Laboratory of Green Chemistry and Chemical Processes, School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, 200062, China.
Copper-based electrocatalysts are recognized as crucial catalysts for CO electroreduction into multi-carbon products. However, achieving copper-based electrocatalysts with adjustable valences via one-step facile synthesis remains a challenge. In this study, Cu/CuO heterostructure is constructed by adjusting the anion species of the Cu ions-containing electrolyte during electrodeposition synthesis.
View Article and Find Full Text PDFRecent Advances and Future Directions in Extracorporeal Carbon Dioxide Removal.
J Clin Med
December 2024
Department of Adult Critical Care, Guy's and St Thomas' NHS Foundation Trust, King's Health Partners, London SE1 9RT, UK.
Extracorporeal carbon dioxide removal (ECCOR) is an emerging technique designed to reduce carbon dioxide (CO) levels in venous blood while enabling lung-protective ventilation or alleviating the work of breathing. Unlike high-flow extracorporeal membrane oxygenation (ECMO), ECCOR operates at lower blood flows (0.4-1.
View Article and Find Full Text PDFSupercritical Extraction and Compound Profiling of Diverse Edible Mushroom Species.
Foods
January 2025
Faculty of Technology Novi Sad, University of Novi Sad, Boulevard cara Lazara 1, 21000 Novi Sad, Serbia.
Mushrooms are a raw material rich in many nutritional compounds, and that is why a number of them are widely known as functional food. They contain fatty acids, carbohydrates, lycopene, sterols, lovastatin, trace elements, and other valuable compounds that show a wide range of properties, such as hepatoprotective, anticancer, antiviral, etc. For more efficient utilisation of mushrooms' biologically active substances, widespread supercritical carbon dioxide extraction (Sc-CO) was used as an efficient way to isolate the high-value phytoconstituents from this type of raw material.
View Article and Find Full Text PDFOxometallate-Based Ionic Liquid Catalyzed CO-Promoted Hydration of Propargylic Alcohols for α-Hydroxy Ketones Synthesis.
Int J Mol Sci
December 2024
State Key Laboratory of Advanced Technology for Materials Synthesis and Processing, Wuhan University of Technology, Wuhan 430070, China.
α-Hydroxy ketones are a crucial class of organic compounds prevalent in natural products and pharmaceutical molecules. The CO-promoted hydration of propargylic alcohols is an efficient method for the synthesis of α-hydroxy ketones. Herein, an ionic liquid (IL) was designed to catalyze this reaction individually under atmospheric CO pressure, volatile organic solvents, and additives.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!