Transmembrane signaling by the high-affinity IgE receptor on membrane preparations.

Proc Natl Acad Sci U S A

Section on Chemical Immunology, National Institute of Arthritis and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, MD 20892.

Published: December 1992

Aggregating the receptor with high affinity for IgE (Fc epsilon RI) stimulates a variety of phenomena in mast cells. Previous efforts to reproduce some of these events in broken-cell preparations such as isolated membranes have had limited success, possibly because the phenomena being monitored were too distal from the initial events. One of the earliest responses is now known to be the phosphorylation of tyrosine residues on several proteins, including the beta and gamma subunits of Fc epsilon RI. We show that in cell sonicates or on partially purified membranes derived from tumor mast cells, aggregating Fc epsilon RI stimulates phosphorylation of receptor tyrosine residues. As in the intact cells, receptor-mediated phosphorylation occurs only on receptors that are themselves aggregated. Because even in the unfractionated sonicates the phosphorylation of other cellular components was not detectably enhanced, and because the evidence is against the receptor itself being a kinase, our results suggest that phosphorylation of Fc epsilon RI is one of the earliest events stimulated by the receptor--an event that can now be investigated on simpler biological preparations than previously available.

Download full-text PDF

Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC50568PMC
http://dx.doi.org/10.1073/pnas.89.23.11446DOI Listing

Publication Analysis

Top Keywords

epsilon stimulates
8
mast cells
8
tyrosine residues
8
phosphorylation
5
transmembrane signaling
4
signaling high-affinity
4
high-affinity ige
4
receptor
4
ige receptor
4
receptor membrane
4

Similar Publications

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!