During larval development of the greater wax moth, Galleria mellonella, genes of storage proteins LHP76 and LHP82 are tissue- and stage-specifically expressed. In this study, hormonal regulation of this expression has been investigated in vivo. Messenger RNAs of the juvenile hormone (JH-suppressible) Lhp82 gene are present only during the feeding period of the final larval instar, suggesting that a high level of JH during earlier stages prevents its expression and that a small rise in JH titer observed on day 8 of the final larval instar is responsible for the rapid shut-off of its transcription. Application of 1micro g of JH analog (fenoxycarb) specifically inhibits expression of Lhp82, whereas Lhp76 mRNAs remain at the same level. 20-hydroxyecdysone (20HE) does not exert any inhibitory effects on transcription of Lhp genes when injected in a dose of 0.5 or 1.5 micro g per individual, regardless of larval age. However, the same dose of 20HE significantly lowers the rate of LHPs synthesis within the fat body and completely blocks secretion of LHPs into the hemolymph. Therefore, we propose that 20HE inhibits the synthesis of storage proteins and their secretion without altering the level of mRNAs.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0022-1910(03)00026-x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Prospects of cowpea protein as an alternative and natural emulsifier for food applications: Effect of pH and oil concentration.
Int J Biol Macromol
January 2025
Department of Food Engineering and Technology, Institute of Biosciences, Humanities and Exact Sciences, São Paulo State University (UNESP), Street Cristóvão Colombo, 2265, São José do Rio Preto 15054-000, Brazil. Electronic address:
In response to the growing need to expand the knowledge base on novel, more sustainable protein sources, this study investigated the effectiveness of cowpea protein concentrate (CPC) as a natural emulsifying agent, examining the relationships between pH (3-11), oil concentration (2-10 %), and emulsion stability. pH and oil concentration significantly impacted droplet size distribution, with uniformity decreasing in the order of pH 9 > pH 11 > pH 7, which was attributed to droplet coalescence and flocculation. As evidenced by circular dichroism, alkalinity induced a slight increase in the beta-sheet content of CPC, while simultaneously reducing the alpha-helix content.
View Article and Find Full Text PDFThe Multidrug and toxin compound extrusion gene GhTT12 promotes the accumulation of both proanthocyanidins and anthocyanins in Gossypium hirsutum.
Plant Physiol Biochem
January 2025
Plant Genomics and Molecular Improvement of Colored Fiber Laboratory, Zhejiang Sci-Tech University, Hangzhou, 310018, China. Electronic address:
The pigments present in the fibers of naturally colored cotton provide excellent antibacterial and environmentally friendly properties, making these colored fibers increasingly favored by the textile industry and consumers. Proanthocyanidins (PAs), the critical pigments responsible for the color of brown cotton fiber, are produced on the endoplasmic reticulum and subsequently transported to the vacuole for polymerization and/or storage. Previous studies have identified GhTT12 as a potential transmembrane transporter of PAs in Gossypium hirsutum, with GhTT12 being a homolog of Arabidopsis Transparent Testa 12 (TT12).
View Article and Find Full Text PDFDevelopment and characterization of quinoa protein-fucoidan emulsion gels with excellent rheological properties for 3D printing and curcumin encapsulation stability.
Food Chem
January 2025
Department of Food Science, College of Food Science and Engineering, Jilin University, Changchun 130062, China. Electronic address:
Using of low-fat emulsion gels stabilized by quinoa protein (QP) for 3D food printing was limited by their defective rheological properties. The study was to explore the feasibility of using fucoidan (FU) to improve the printability and curcumin encapsulation stability of QP emulsion gel. The gels with 0.
View Article and Find Full Text PDFProtein-Guided Biomimetic Calcification Constructing 3D Nitrogen-Rich Core-Shell Structures Realizing High-Performance Lithium-Sulfur Batteries.
Adv Mater
January 2025
School of Chemistry and Chemical Engineering, Yangzhou University, Yangzhou, Jiangsu, 225009, P. R. China.
Biomimetic calcification is a micro-crystallization process that mimics the natural biomineralization process, where biomacromolecules regulate the formation of inorganic minerals. In this study, it is presented that a protein-assisted biomimetic calcification method for the in situ synthesis of nitrogen-doped metal-organic framework (MOF) materials. A series of unique core-shell structures are created by utilizing proteins as templates and guiding agents in the nucleation step, creating ideal conditions for shell growth.
View Article and Find Full Text PDFL. Oligo-Polysaccharides (CIO) Exerts Antianxiety and Antidepressant Effects on Mice Experiencing Behavioral Despair and Chronic Unpredicted Mild Stress.
Foods
January 2025
Research Center for Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China.
L. oligo-polysaccharides (CIOs), obtained from L., is a mixture of oligosaccharides and polysaccharides.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!