Detection of Chlamydophila felis and feline herpesvirus by multiplex real-time PCR analysis.

J Clin Microbiol

Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol BS40 5DU, United Kingdom.

Published: June 2003

Chlamydophila felis and feline herpesvirus (FHV) are pathogens commonly associated with feline respiratory and ocular disease. A real-time multiplex PCR assay was developed to allow detection of these organisms, together with feline 28S ribosomal DNA, in a single tube. Of 538 ocular swab samples tested, 123 were positive for FHV, 97 were positive for C. felis, and 16 were positive for both pathogens.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC156487PMC
http://dx.doi.org/10.1128/JCM.41.6.2734-2736.2003DOI Listing

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