AI Article Synopsis
- The study focuses on the Drosophila mitochondrial transcription factor A (D-mtTFA), detailing its cloning and characterization.
- D-mtTFA mRNA and protein levels are high in cells that are dividing and undergoing a specific type of cell growth.
- The findings indicate that D-mtTFA is involved in mitochondrial functions as well as contributing to cell growth and development, particularly in the context of spermatogenesis.
Article Abstract
We previously reported cloning of the Drosophila mitochondrial transcription factor A (D-mtTFA) gene, and characterization of a recombinant protein. In this report, the expression and distribution patterns of D-mtTFA during development are described. D-mtTFA mRNA and its protein product were found to be abundant in cells of tissues undergoing both proliferation and polytenization. Furthermore, a DRE-like sequence present in the D-mtTFA gene showed promotor activity in Drosophila Kc cells. In addition, D-mtTFA was detected in brain throughout the developmental process, as well as in non-proliferating tissues, such as flight muscle and cardia, and was also found in spermatids of imagos. D-mtTFA possesses a nuclear-targeting sequence, and is present in the nucleus at the syncytial stage, where bundles of 64 spermatids are present during spermatogenesis. The results suggest that D-mtTFA not only contributes to the mitochondrial DNA transcription and replication system, but also has a role in cell proliferation and development, including spermatogenesis.
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| http://dx.doi.org/10.1016/s1065-6995(02)00355-4 | DOI Listing |
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