Objective: To determine the content of imperatorin in the tetraploidy radix angelicae dahuricae, and compare it with the original diplontic varites.
Method: The chromatographic method was carried out on Nova-pak (4.6 mm x 200 mm, 5 microns) column with acetonitrile-water solution as a mobile phase. The flow rate was 1 ml.min-1. the detection wavelength was at 248 nm, and the column temperature was 25 degrees C.
Result: The eontent of was imperatorin in the tetraploidy radix 0.460% and 0.225% imperatorin in the diplontic species.
Conclusion: The content of the mainly active constituent in the tetraploid is double to what it is in the original diplontic species.
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