Low-power photographs of brain sections have been needed for neuroanatomical studies. This paper describes a setup for fluorescence digital photomacrography. High-resolution digital images were obtained with commercially available digital cameras combined with an oblique fluorescence illumination and filters for excitation and emission. The image of an entire coronal section of the macaque brain could be taken with a single exposure. The present photographic system would be useful not only for neuroanatomical, but also for histological, embryological or pathological studies that require low-magnification fluorescence images.
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http://dx.doi.org/10.1016/s0165-0270(03)00021-9 | DOI Listing |
Biotechnol Bioeng
January 2025
State Key Laboratory of Bioreactor Engineering, Qingdao Innovation Institute of East China University of Science and Technology, East China University of Science and Technology, Shanghai, China.
High-performance strain and corresponding fermentation process are essential for achieving efficient biomanufacturing. However, conventional offline detection methods for products are cumbersome and less stable, hindering the "Test" module in the operation of "Design-Build-Test-Learn" cycle for strain screening and fermentation process optimization. This study proposed and validated an innovative research paradigm combining computer vision with deep learning to facilitate efficient strain selection and effective fermentation process optimization.
View Article and Find Full Text PDFACS Nano
January 2025
School of Life and Health Sciences, Hainan Province Key Laboratory of One Health, Collaborative Innovation Center of One Health, Hainan University, Haikou, Hainan 570228, China.
Treatment of tumor brain metastases remains challenging due to the ineffectiveness of drugs in crossing the blood-brain barrier (BBB). Here, we proposed a potential strategy to target and modulate the meningeal lymphatic system for immunotherapy of breast cancer brain metastases (BCBM) through peripheral administration. CT/fluorescence dual-modality imaging demonstrated that the phospholipid nanoprobe (α-PLNPs) through intracisternal magna injection effectively labeled and long-range tracked the meningeal lymphatic pathway from meningeal lymphatic vessels (MLVs) to periphery drainage cervical lymph nodes (CLNs).
View Article and Find Full Text PDFFood Chem X
January 2025
College of Food Science and Engineering, Shanxi Agricultural University, Taiyuan, Shanxi, China.
Aiming to enable online freshness-monitoring of meat within modified-atmosphere package, we developed a ratiometric array that was fluorescently responsive to volatile organic compounds-ammonia (NH) released by protein decaying. The array was consisted of two 3 mm × 6 mm rectangles precisely and uniformly printed with fluorescein isothiocyanate (FITC) as indicator and rhodamine B (RhB) as internal reference on the filter-paper, respectively. The fluorescence intensity of the array area was calibrated according to Green/Red ratio of the digitalized pixels extracted from images facilitated by a smartphone.
View Article and Find Full Text PDFAnal Chem
January 2025
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200030, China.
Multiplex digital nucleic acid analysis (NAA) allows the precise quantification of multiple target nucleic acids with single-molecule sensitivity, making it highly appealing for life science research and clinical diagnostics. Nucleic acid-guided endonucleases, such as CRISPR, have demonstrated great potential in digital NAA. However, performing multiplex digital NAA with an endonuclease remains challenging.
View Article and Find Full Text PDFInt J Oral Sci
January 2025
State Key Laboratory for Diagnosis and Treatment of Severe Zoonotic Infectious Diseases, Key Laboratory for Zoonosis Research of the Ministry of Education, College of Basic Medical Science, Jilin University, Changchun, China.
Tongue squamous cell carcinoma (TSCC) is a prevalent malignancy that afflicts the head and neck area and presents a high incidence of metastasis and invasion. Accurate diagnosis and effective treatment are essential for enhancing the quality of life and the survival rates of TSCC patients. The current treatment modalities for TSCC frequently suffer from a lack of specificity and efficacy.
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