AI Article Synopsis
- The protein lysyl-tRNA synthetase (LysRS) has two forms: class I and class II, with Borrelia burgdorferi having the class I type while its hosts have class II.
- Researchers cloned and sequenced the lysK gene from several Borrelia species to explore its potential as a diagnostic target for Lyme disease.
- Using these sequences, a primer set was developed for detecting and genotyping different Borrelia species in one PCR test, ensuring specificity by avoiding cross-reactivity with other bacteria.
Article Abstract
The essential protein lysyl-tRNA synthetase (LysRS) exists in two unrelated forms, a class I and a class II-type aminoacyl-tRNA synthetase. Comparative genome sequence analysis revealed that Borrelia burgdorferi sensu lato, the etiological agent of Lyme disease, contains a class I-type LysRS, whereas its tick and mammalian hosts would be expected to contain a class II-type protein. To investigate the utility of the class I LysRS as a diagnostic target for Lyme disease, the corresponding gene ( lysK) was cloned and sequenced from B. afzelii, B. garinii, and B. hermsii. These lysK sequences were then used to design a primer set that could detect and genotype B. burgdorferisensu strictu, B. afzelii, and B. garinii in one single polymerase chain reaction, while showing no cross reactivity with examples of other Borrelia or spirochetes.
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| http://dx.doi.org/10.1007/s00430-002-0149-7 | DOI Listing |
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