In exploratory scale biotechnological process development, the product must be rapidly identified although a reference compound may not always be available. LC-diode-array detection and MS were used for this purpose in a process producing 9alpha-hydroxyprogesterone from progesterone as substrate. The electrospray ionization mass spectrometer was combined with an ion trap mass spectrometer for the second generation MS. The preliminary identification, which could be carried out within the course of a day, confirmed that the product was a hydroxyprogesterone. The final identification step, which was much more material intensive and hence time consuming, involved a two-step preparative separation to yield quantities necessary for definitive product identification based on 1H- and 13C NMR.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0021-9673(03)00314-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Optimizing Correction Factors on Color Differences for Automotive Painting Services.
Sensors (Basel)
December 2024
Department of Management and Industrial Engineering, University of Petrosani, 332003 Petrosani, Romania.
Currently, the automotive sector is showing increased demands regarding the color of cars in general, but especially the quality and the time of painting, in particular. Companies working in this industry, especially in specialized painting services, must perform work of impeccable quality in the shortest possible time in order to be efficient. Color differences that appear in different areas of the car result from the use of different formulas for obtaining color.
View Article and Find Full Text PDFResearch on Parameter Compensation Method and Control Strategy of Mobile Robot Dynamics Model Based on Digital Twin.
Sensors (Basel)
December 2024
School of Mechanical Engineering and Automation, Foshan University, Foshan 528225, China.
Inspection robots, which improve hazard identification and enhance safety management, play a vital role in the examination of high-risk environments in many fields, such as power distribution, petrochemical, and new energy battery factories. Currently, the position precision of the robots is a major barrier to their broad application. Exact kinematic model and control system of the robots is required to improve their location accuracy during movement on the unstructured surfaces.
View Article and Find Full Text PDFBlade Vibration Difference-Based Circumferential Fourier Fitting Algorithm for Synchronous Vibration Parameter Identification of Rotation Blades.
Sensors (Basel)
December 2024
School of Data Science and Technology, North University of China, Taiyuan 030051, China.
Blades are the core components of rotating machinery, and the blade vibration status directly impacts the working efficiency and safe operation of the equipment. The blade tip timing (BTT) technique provides a solution for blade vibration monitoring and is currently a prominent topic in research on blade vibration issues. Nevertheless, the non-stationary factors present in actual engineering applications introduce inaccuracies in the BTT technique, resulting in blade vibration measurement errors.
View Article and Find Full Text PDFInvestigation of Separating Temperature-Induced Structural Strain Using Improved Blind Source Separation (BSS) Technique.
Sensors (Basel)
December 2024
The State Key Laboratory for the Safety, Long-Life, Health Operation and Maintenance of Long-Span Bridges, Jiangsu Provincial Institute of Traffic Science (JSTI Group), Nanjing 210098, China.
The strain data acquired from structural health monitoring (SHM) systems of large-span bridges are often contaminated by a mixture of temperature-induced and vehicle-induced strain components, thereby complicating the assessment of bridge health. Existing approaches for isolating temperature-induced strains predominantly rely on statistical temperature-strain models, which can be significantly influenced by arbitrarily chosen parameters, thereby undermining the accuracy of the results. Additionally, signal processing techniques, including empirical mode decomposition (EMD) and others, frequently yield unstable outcomes when confronted with nonlinear strain signals.
View Article and Find Full Text PDFDiscovery of Non-Peptide GLP-1 Positive Allosteric Modulators from Natural Products: Virtual Screening, Molecular Dynamics, ADMET Profiling, Repurposing, and Chemical Scaffolds Identification.
Pharmaceutics
December 2024
Department of Chemistry, College of Science, Imam Abdulrahman Bin Faisal University, P.O. Box 1982, Dammam 31441, Saudi Arabia.
Glucagon-like peptide-1 (GLP-1) receptor is currently one of the most explored targets exploited for the management of diabetes and obesity, with many aspects of its mechanisms behind cardiovascular protection yet to be fully elucidated. Research dedicated towards the development of oral GLP-1 therapy and non-peptide ligands with broader clinical applications is crucial towards unveiling the full therapeutic capacity of this potent class of medicines. This study describes the virtual screening of a natural product database consisting of 695,133 compounds for positive GLP-1 allosteric modulation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!