Six extracellular yeast glycoproteins were prepared from three yeast species in osmotic equilibrium and unequilibrium environments and used as non-penetrating cryoadditives. Glycoproteins secreted by the strain Dipodascus australiensis into growth medium containing NaCl (8% w/v) were found to be the most effective cryoadditives. It was possible to use these glycoproteins alone (without DMSO as penetrating agent) for the cryoprotection of the studied yeasts.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC140111 | PMC |
| http://dx.doi.org/10.1251/bpo1 | DOI Listing |
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