AI Article Synopsis
- Eleven labs tested dried blood and plasma spots for measuring HIV RNA using two RNA assays: Roche Amplicor and bioMerieux NucliSens.
- Both assays showed a consistent recovery of HIV RNA from 4,000 to 500,000 copies/ml, with Roche being more sensitive at lower levels.
- Dried samples remain stable for a year at room temperature or -70°C, making them practical for field use in diagnosing HIV and monitoring treatment.
Article Abstract
Eleven laboratories evaluated the use of dried blood and plasma spots for quantitation of human immunodeficiency virus (HIV) RNA by two commercially available RNA assays, the Roche Amplicor HIV-1 Monitor and the bioMerieux NucliSens HIV-1 QT assays. The recovery of HIV RNA was linear over a dynamic range extending from 4,000 to 500,000 HIV type 1 RNA copies/ml. The Monitor assay appeared to have a broader dynamic range and seemed more sensitive at lower concentrations. However, the NucliSens assay gave more consistent results and could be performed without modification of the kit. HIV RNA was stable in dried whole blood or plasma stored at room temperature or at -70 degrees C for up to 1 year. Dried blood and dried plasma spots can be used as an easy and inexpensive means for the collection and storage of specimens under field conditions for the diagnosis of HIV infection and the monitoring of antiretroviral therapy.
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Source |
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC154666 | PMC |
| http://dx.doi.org/10.1128/JCM.41.5.1888-1893.2003 | DOI Listing |
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