We previously observed secretion of active-form transglutaminase in Corynebacterium glutamicum by coexpressing the subtilisin-like protease SAM-P45 from Streptomyces albogriseolus to process the prodomain. However, the N-terminal amino acid sequence of the transglutaminase differed from that of the native Streptoverticillium mobaraense enzyme. In the present work we have used site-directed mutagenesis to generate an optimal SAM-P45 cleavage site in the C-terminal region of the prodomain. As a result, native-type transglutaminase was secreted.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC154552 | PMC |
| http://dx.doi.org/10.1128/AEM.69.5.3011-3014.2003 | DOI Listing |
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