To identify genes that are associated with progression of malignant lymphoma, the expression profiles of 18,432 genes were analyzed in diffuse large B-cell lymphomas at early (stages I and II, 6 cases) and advanced stages (stages III and IV, 9 cases) by means of cDNA microarrays. By comparing expression profiles between localized and advanced lymphomas, a number of genes that were differentially expressed were identified: 48 genes with increased expression and 30 genes with reduced expression in advanced-stage diffuse large B-cell lymphomas. Increased expression of MPHOSPH1, RUVBL1, CHN2, PSA and CDC10 genes, and reduced expression of COL1A2, COL4A1, FBLN5, CLECSF6, MIC2, CAV1 and S100A10 genes in the advanced lymphoma group were confirmed by semi-quantitative reverse transcription-PCR. RUVBL1 and PSA expression was further confirmed by real-time quantitative PCR, whose results paralleled the microarray data. The highly expressed genes encode proteins that promote cell proliferation and the genes with reduced expression encode adhesion proteins and target protein for cytotoxic T-lymphocytes. These findings suggested that analysis with cDNA microarrays is a useful approach for identifying genes related to tumor progression and their products could be potential tumor markers or disease-specific targets for anti-tumor therapy.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5927108 | PMC |
| http://dx.doi.org/10.1111/j.1349-7006.2002.tb01335.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Protein molecular structures of USP53, NPY2R, and DCTN1-AS1 and impact on tumors: Analysis of prognostic biomarkers for diffuse large B-cell lymphoma.
Int J Biol Macromol
January 2025
Department of Radiotherapy, Harbin Medical University Cancer Hospital, Harbin Medical University, Harbin, Heilongjiang 150081, China. Electronic address:
In the past few years, three protein molecules-USP53, NPY2R, and DCTN1-AS1-have garnered significant attention in scientific research due to their potential implications in tumor development. Mass spectrometry and proteomics techniques were used to analyze the three-dimensional structure of these protein molecules and predict their active sites and functional domains. The effects of USP53, NPY2R and DCTN1-AS1 on biological behavior of tumor cells were studied by constructing gene knockout and overexpression cell models.
View Article and Find Full Text PDFCDK1 inhibitor RO-3306 enhances BTKi potency in diffuse large B-cell lymphoma by suppressing JAK2/STAT3 signaling.
Int J Biol Macromol
January 2025
Department of Hematology, The Affiliated Huaian No.1 People's Hospital of Nanjing Medical University, Huaian 223300, Jiangsu Province, PR China; Key Laboratory of Hematology of Nanjing Medical University, Nanjing 210029, Jiangsu Province, PR China. Electronic address:
Diffuse large B-cell lymphoma (DLBCL) is the most common type of lymphoma in adults, which characterized by a high degree of heterogeneity in terms of clinical presentation, molecular phenotype, and genetic features. However, approximately 30 %-40 % of patients are refractory to standard chemotherapy, and their prognosis is poor. The emergence of small-molecule inhibitors, such as Bruton's tyrosine kinase inhibitors (BTKi), has greatly improved the treatment of DLBCL; however, drug resistance associated with small-molecule inhibitors has greatly limited their clinical application.
View Article and Find Full Text PDFOff-axis electron holography of unstained bacteriophages: Toward electrostatic potential measurement of biological samples.
J Struct Biol
January 2025
CEMES-CNRS, Université de Toulouse, I3EM Team, 29 rue JeanneMarvig B.P, 94347 31055 Toulouse, France. Electronic address:
Transmission electron microscopy, especially at cryogenic temperature, is largely used for studying biological macromolecular complexes. A main difficulty of TEM imaging of biological samples is the weak amplitude contrasts due to electron diffusion on light elements that compose biological organisms. Achieving high-resolution reconstructions implies therefore the acquisition of a huge number of TEM micrographs followed by a time-consuming image analysis.
View Article and Find Full Text PDFPET-based lesion graphs meet clinical data: An interpretable cross-attention framework for DLBCL treatment response prediction.
Comput Med Imaging Graph
December 2024
Nantes Université, Centrale Nantes, CNRS, LS2N, UMR 6004, F-44000 Nantes, France.
Diffuse Large B-cell Lymphoma (DLBCL) is a lymphatic cancer of steadily growing incidence. Its diagnostic and follow-up rely on the analysis of clinical biomarkers and 18F-Fluorodeoxyglucose (FDG)-PET/CT images. In this context, we target the problem of assisting in the early identification of high-risk DLBCL patients from both images and tabular clinical data.
View Article and Find Full Text PDFIdentification and treatment of false methane values produced by the tunable diode laser absorption spectroscopy technology equipped on unmanned aerial vehicles.
Integr Environ Assess Manag
January 2025
Ionian Department, University of Bari Aldo Moro, Bari, Italy.
Fugitive or diffuse methane emissions constitute an important source of damage to the environment, much greater even than CO2 both over a time span of 20 years and over a longer time span of 100. It is therefore of preeminent importance to undertake all the efforts necessary to implement new tools, protocols, and methods that contribute to the identification and measurement of these emissions to implement site-specific actions of mitigation, repair, and conscious management of the emitting plants. Among the remote sensing and leak detection technologies currently used, the tunable diode laser absorption spectroscopy (TDLAS) method plays a relevant role.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!