An evaluation of the performance of the GEN-S analyzer in examining the parameters of reticulocytes is described. The automated and manual methods of counting the relative quantity of reticulocytes were compared. It was established that the precision of the analyzer significantly surpasses the manual method results. The variation coefficient for the parameters of examination of reticulocytes is lower at a higher content of reticulocytes and the accuracy of the method is better if the content of reticulocytes in blood is higher. The parameters, characterizing the fraction of immature reticulocytes, are least accurate. The study demonstrated that the automated method of analyzing the reticulocytes by using the GEN-S analyzer can be used to ensure a more accurate monitoring of changes of the function of erythropoesis at different anemic conditions and to assess the conducted therapy.
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Background: The aim of the study was to perform an internal comparison of blood cell analyses: white blood cell (WBC) and platelet (PLT) counts in a clinical laboratory.
Methods: Instrument comparison: Based on EP9-A2 of the Clinical Laboratory and Standard Institute (CLSI) and the statistics program of External Quality Assessment (EQA), we set 1/2CLIA'88 as our quality standard and used a Beckman coulter LH750 as the reference. We then compared this to another 4 blood analyzers: the Beckman Coulter Gen-S, ABX pentra 60, and the Sysmex XT2000i and XS-800i, including the Beckman Coulter Gen-S in manual mode.
Aim: To show distribution of the investigated patients into diagnostic groups, find out the diagnostic value of the levels of hemoglobin and packed cell volume as possible markers of absolute erythrocytosis in the group of patients with polycythaemia.
Material And Methods: We evaluated 61 patients, mean age was 46 years (18-82), 9 females and 52 males before treatment. Mean levels of hemoglobin in females - 171 g\l (143-190), packed cell volume 52% (49-61).
J Pak Med Assoc
November 2010
Section of Haematology, Department of Pathology and Microbiology, The Aga Khan University, Karachi, Pakistan.
Objectives: To establish the credibility of manual reticulocyte counts without compromising the quality of care, and to evaluate the degree of acceptability of manual reticulocyte counts in terms of accuracy and cost effectiveness in comparison with two automated haematology analyzers.
Methods: Visual reticulocyte enumeration was evaluated for comparability, within-batch precision and costing with respect to Coulter STKS and Gen S haematology analyzers.
Results: The results of reticulocyte estimation for 80 samples as obtained by 3 modes were correlated using Pearson's correlation coefficient (r) which were computed as 0.
Malar J
November 2010
Grupo Malaria, Facultad de Medicina, Universidad de Antioquia, Calle 62 #52-59, Lab 610, Medellin, Colombia.
For more than a decade, flow cytometry-based automated haematology analysers have been studied for malaria diagnosis. Although current haematology analysers are not specifically designed to detect malaria-related abnormalities, most studies have found sensitivities that comply with WHO malaria-diagnostic guidelines, i.e.
View Article and Find Full Text PDFAnn Biol Clin (Paris)
December 2008
Laboratoire d'hématologie, CHU Caremeau, Nîmes.
Excell 2280 analyser is a new automated haematology analyser manufactured by Drew Scientific Inc, Texas, USA, and distributed in France by MAXMAT S.A., Montpellier.
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