AI Article Synopsis
- The research developed new anti-mannose receptor (MR) monoclonal antibodies to study MR expression in mouse macrophages, which was previously challenging due to the lack of specific tools.
- The study found that interleukin (IL)-4 increased the total and surface levels of MR in macrophages, while IL-10, which typically reduces macrophage function, had a similar effect.
- Additionally, the research revealed the presence of soluble MR (sMR) in non-macrophage cells, suggesting that the process of MR cleavage and sMR production is not limited to macrophages.
Article Abstract
The study of the murine macrophage mannose receptor (MR) has been hampered by the lack of specific reagents. We have generated and characterized novel anti-MR monoclonal antibodies and used them to analyze MR expression in primary mouse macrophages (M(phi)). In BioGel- and thioglycollate-elicited M(phi), interleukin (IL)-4 up-regulated total cell-associated MR (cMR), correlating with enhanced surface expression. We investigated the influence of IL-10, a well-characterized deactivator of M(phi) function, on MR levels and observed that it had a similar effect to IL-4. In both cases, enhanced cMR levels translated into increased production of the soluble form of the receptor (sMR). We have demonstrated the presence of sMR in cultures of stable non-M(phi) transductants expressing full-length MR, indicating that the proteolytic activity responsible for cMR cleavage is not M(phi)-restricted. These data support a role for the MR in T helper cell type 2 cytokine-driven, immune responses and suggest a non-M(phi) contribution to sMR production in vivo.
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| http://dx.doi.org/10.1189/jlb.0902450 | DOI Listing |
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