[Quantitative determination of 3-nitropropionic acid by HPLC].

A reversed-phase liquid chromatographic method to determine the concentration of 3-nitropropionic acid (3-NPA) in the culture medium with Aspergillus oryzae was developed. The culture medium samples were extracted with ethyl acetate. The analytic column was Hypersil C18 and the mobile phase was CH3CN/KH2PO4(0.02 mol/L, pH 3.0) (1/3, v/v). The sample was detected at UV210 nm. The detection limit of this method was at 10 micrograms/kg level and recoveries were 90.1%-98.0.