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Heme oxygenase expression in selected regions of term human placenta. | LitMetric

Heme oxygenase expression in selected regions of term human placenta.

Exp Biol Med (Maywood)

Departments of Pharmacology, Faculty of Health Sciences, Queen's University, Kingston, Ontario, Canada K7L 3N6.

Published: May 2003

AI Article Synopsis

  • This study examines the role of carbon monoxide in regulating blood flow in the placenta by analyzing heme oxygenase (HO) activity and protein levels in different regions of the placenta from both normal and mild pre-eclamptic pregnancies.
  • Researchers measured HO activity through gas chromatography and assessed the levels of HO-1 and HO-2 proteins using Western immunoblot analysis.
  • Findings showed no difference in overall HO activity or HO-2 levels between the two groups, but there was an increase in HO-1 protein in certain placental areas of pre-eclamptic pregnancies, indicating a potential adaptive response to stress.

Article Abstract

Carbon monoxide (CO), formed during heme oxygenase (HO)-catalyzed oxidation of heme, has been proposed to play a complementary role with nitric oxide in the regulation of placental hemodynamics. The objective of this study was to elucidate HO enzymatic activity and HO-1 (inducible) and HO-2 (constitutive) protein content in the microsomal subcellular fraction of homogenate of selected regions of placenta from normotensive and mild pre-eclamptic pregnancies. HO enzymatic activity was measured under optimized conditions by gas chromatography using CO formation as an index of activity, and HO-1 and HO-2 protein content were determined by Western immunoblot analysis. Microsomal HO activity in each of the four placental regions was not different between normotensive and mild pre-eclamptic pregnancies. Microsomal HO-2 protein content was not different between normotensive and mild pre-eclamptic pregnancies, whereas there was increased expression of microsomal HO-1 protein in chorionic villi and fetal membranes from pre-eclamptic pregnancy compared with normotensive pregnancy. Microsomal HO enzymatic activity correlated with HO-2, but not HO-1, protein content.

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Source
http://dx.doi.org/10.1177/15353702-0322805-28DOI Listing

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