To deal with the complexity of the glycan mixtures released from glycoproteins, an efficient form of micro-column separations, capillary electrochromatography, has been combined with high-performance mass spectrometry (Fourier transform ion cyclotron resonance). Contour plots have been generated from the mixtures of O-linked oligosaccharides originated from bovine mucin and bile salt-stimulated lipase, a large glycoprotein enzyme.
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http://dx.doi.org/10.1021/ac025985c | DOI Listing |
Anal Chim Acta
November 2024
Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, Nanjing, 210009, PR China; State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, 210009, PR China. Electronic address:
Background: Chirality is one of the most fundamental features of nature. In terms of biological activities, pharmacological effects, etc., enantiomers often show great differences among each other.
View Article and Find Full Text PDFSe Pu
November 2024
College of Materials and Chemical Engineering, Minjiang University, Fuzhou 350108, China.
Metal organic frameworks (MOFs) are crystalline compounds composed of metal ions (or metal clusters) and organic ligands. Chiral MOFs have been successfully utilized as novel materials for the separation of chiral enantiomers by chromatography, demonstrating excellent chiral separation performance. In this study, a chiral MOF-modified silica monolithic capillary column was used for pressurized capillary electrochromatography.
View Article and Find Full Text PDFMikrochim Acta
October 2024
Key Laboratory of Drug Quality Control and Pharmacovigilance (Ministry of Education), China Pharmaceutical University, No.24 Tongjiaxiang, Nanjing, Jiangsu, 210009, P. R. China.
A capillary electrochromatography (CEC) synergistic enantioseparation system based on a novel nanomaterial synthesized by chiral molecularly imprinted polymers (CMIPs) and chiral metal organic frameworks (CMOFs) was developed. Compared with CMIPs and CMOFs alone, the enantioseparation performance of ofloxacin (OFL) of the CEC with the novel nanomaterial as stationary phases was greatly improved. CMOFs with chiral recognition ability have synergize with CMIPs to greatly improve the chiral selectivity of the novel stationary phases in CEC.
View Article and Find Full Text PDFJ Chromatogr A
November 2024
School of Pharmacy, Southwest Medical University, Luzhou, 646000, China. Electronic address:
In recent years, mussel-inspired polydopamine (PDA)-based materials have attracted significant attention in the field of open-tubular capillary electrochromatography (OT-CEC) owing to their diverse and appealing properties. However, previously established functionalized PDA coating-based CEC stationary phases predominantly relied on the latent reactivity of PDA with amine/thiol-containing molecules, limiting the types of applicable modifiers and requiring time-consuming reaction processes. Herein, we presented a versatile and efficient method for the facile and rapid fabrication of diverse functionalized PDA coatings as OT-CEC stationary phases through a Zr(IV) coordination-mediated post-modification strategy.
View Article and Find Full Text PDFJ Chromatogr A
November 2024
Key Laboratory for Analytical Science of Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou 350116, China; International (HongKong Macao and Taiwan) Joint Laboratory on food safety and environmental analysis, Fuzhou 350116, Fuzhou University, China. Electronic address:
The occurrence of microcystins (MCs) during harmful algal blooms (HABs) represents a major threat to freshwater environments. In this work, a novel surface amphiphilic hybrid porous polymers based on cage-like organosiloxanes (PCSs) was prepared for the enrichment of MCs. The copolymerization of bifunctional amphiphilic monomers, 2-methacryloyloxyethyl phosphorylcholine (MPC) and N-benzylquininium chloride (BQN), with the cross-linker methacryl substituted polyhedral oligomeric silsesquioxane (POSS) was achieved in an ionic liquid-based porogenic medium.
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