The roots of Acanthopanax senticosus (Rupr. et Maxim.) Harm were obtained for the acquisition of the crude extractives to isolate the 19 components by means of preliminary extraction and isolation. High-performance liquid chromatography followed by primary and secondary mass spectrum analysis (HPLC/MS/MS) was employed to identify the antifatigue components from the extracts of the plant with reference to the literature. Good isolation results were achieved and several known and unknown chemical compounds were identified, for instance, in one sample (No. 11) 5 known components were detected along with 6 unknown compounds with relative molecular mass of 302, 318, 346, 354, 390, and 406, respectively.
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