AI Article Synopsis
- The study focused on the rat submaxillary gland to find GnRH and its receptors, using methods like immunohistochemistry and RT-PCR.
- Findings indicated that both GnRH and its receptors were found together in the gland's epithelial cells, suggesting they are localized in the same areas.
- The results imply that the rat submaxillary gland can produce GnRH and its receptors, which might play a role in regulating the gland's functions through local signaling mechanisms.
Article Abstract
We investigated the rat submaxillary gland for the presence of GnRH and GnRH receptors, the localization and colocalization of GnRH, GnRH receptor and their mRNA, and studied the sequence of GnRH receptor complementary DNA (cDNA) by immunohistochemistry, in situ hybridization and RT-PCR. The results showed that GnRH and GnRH receptor immunoreactive materials were colocalized in the epithelial cells of the serous acinus and glandular duct. The GnRH and GnRH receptor mRNA hybridization signals were detected in the above cells. The sequence obtained from the RT-PCR product was identical to the published cDNA sequence of GnRH receptor in the rat pituitary. The results suggested that the rat submaxillary gland was capable of synthesizing GnRH and GnRH receptors. GnRH may be involved in the functional regulation of the submaxillary gland through autocrine or paracrine activity.
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| http://dx.doi.org/10.1016/s0024-3205(03)00194-2 | DOI Listing |
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