A new apigeninglycoside, apigenin 6-C-galactosyl-6"-O-beta-galactopyranoside (1), isoorientin, and a mixture of orientin and isovitexin were isolated from leaves of Cecropia lyratiloba by high-speed countercurrent chromatography using a solvent system containing ethyl acetate, butanol, methanol and water. The structural elucidation of 1 was based on NMR spectroscopy.
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http://dx.doi.org/10.1002/pca.688 | DOI Listing |
Molecules
December 2024
School of Pharmacy and Pharmaceutical Sciences, Panoz Institute, and Trinity Biomedical Sciences Institute, Trinity College Dublin, Dublin 2, D02 PN40 Dublin, Ireland.
is a lesser-known plant species belonging to the genus that grows in Kazakhstan. The aim of this study was to characterize the composition of the ethanolic, water, and hydroethanolic extracts from the aerial parts of by HPLC-ESI-QTOF-MS/MS to isolate the major compound isoquercitrin by HSCCC (High-Speed Counter-Current Chromatography) and to determine the cytotoxicity and anti-inflammatory potential of the extracts produced with this plant. Fingerprinting of the analyzed extracts showed the presence of a multitude of metabolites comprising polyphenols, organic acids, and coumarins, and only trace quantities of cardiac glycosides in the analyzed samples.
View Article and Find Full Text PDFJ Sep Sci
December 2024
CAS Key Laboratory of Chemistry of Northwestern Plant Resources and Key Laboratory of Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences (CAS), Lanzhou, China.
Natural products play a vital role in human health because people find that they can reduce the incidence of many diseases, such as cancer and cardiovascular diseases. Therefore, researchers have developed many effective techniques for screening and separating active compounds from natural products. High-speed countercurrent chromatography is a unique unsupported liquid-liquid chromatography technology that has been widely used in the separation of natural products.
View Article and Find Full Text PDFJ Sep Sci
December 2024
College of Science, Gansu Agricultural University, Lanzhou, China.
In this work, different injection modes (single injection, overlapping injection, and continuous injection) of countercurrent chromatography were used and compared for the isolation and purification of glabridin from the crude extract of Glycyrrhiza glabra. The two-phase solvent system consisting of n-hexane/ethyl acetate/methanol/water (5:4:5:4, v/v) was employed as stationary and mobile phases. Compared with single injection mode, both overlapping injection and continuous injection modes exhibited higher separation efficiency at the same sample loading capacity.
View Article and Find Full Text PDFPlants (Basel)
October 2024
Laboratório de Produtos Naturais (TecBio), Farmanguinhos, FIOCRUZ Foundation, Rua Sizenando Nabuco 100, Rio de Janeiro 21041-250, RJ, Brazil.
Prep Biochem Biotechnol
October 2024
Laboratório de Produtos Naturais e Ensaios Biológicos (LaProNEB), Departamento de Produtos Naturais e Alimentos, Faculdade de Farmácia, Centro de Ciências da Saúde, Universidade Federal do Rio de Janeiro, Ilha do Fundão, Rio de Janeiro-RJ, Brazil.
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