AI Article Synopsis
- Researchers combined a specific sequence from the foot-and-mouth disease virus with three internal ribosome entry segments to create three distinct pentacistronic retroviruses of varying sizes.
- The new virus vectors successfully co-expressed five proteins efficiently, achieving comparable production levels to shorter genome vectors.
- However, longer vectors than the wild-type virus experienced frequent deletions, impacting their stability.
Article Abstract
The picornavirus foot-and-mouth disease virus 2A sequence was combined with three different internal ribosome entry segments to construct and characterize three independent pentacistronic retroviruses of different sizes. Efficient co-expression of the five proteins was successful and titres obtained for these pentacistronic virus vectors (final genome size approximately 7.9 kb) were comparable to those of vector systems with shorter genomes. Other vectors constructed that exceeded the genome length of the wild-type virus suffered frequent deletions.
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| http://dx.doi.org/10.1099/vir.0.18998-0 | DOI Listing |
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