Sulfoacetaldehyde, an intermediate of interest to those studying microbial metabolism of sulfonates, is commonly synthesized as the bisulfite adduct. A published method presumed to convert this to the free aldehyde (and cited several times elsewhere in the literature) has been shown to be ineffective; this had not been realized by its users because the enzymes under study recognize the adduct as a substrate.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0167-7012(02)00254-3 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Promoted read-through and mutation against pseudouridine-CMC by an evolved reverse transcriptase.
Commun Biol
January 2025
Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, MA, USA.
Pseudouridine (Ψ) is an abundant RNA chemical modification that plays critical biological functions. Current Ψ detection methods are limited in identifying Ψs at base-resolution in U-rich sequence contexts, where Ψ occurs frequently. Here we report "Mut-Ψ-seq" that utilizes the classic N-cyclohexyl N'-(2-morpholinoethyl)carbodiimide (CMC) agent and an evolved reverse transcriptase ("RT-1306") for Ψ mapping at base-resolution.
View Article and Find Full Text PDFFatty aldehyde bisulfite adducts as a purification handle in ionizable lipid synthesis.
RSC Adv
August 2024
BioVectra Inc. Charlottetown Prince Edward Island C1E 0A1 Canada
Article Synopsis
- * Impurities are removed through the formation of a fatty aldehyde bisulfite adduct and the subsequent reductive amination process, which avoids the need for complex separation steps.
- * The approach also applies to other important lipids like FTT5 and SM-102, aiming to create a more sustainable supply chain for essential vaccines and medicines globally.
Promoted Read-through and Mutation Against Pseudouridine-CMC by an Evolved Reverse Transcriptase.
bioRxiv
July 2024
Department of Chemistry, Merkert Chemistry Center, Boston College, Chestnut Hill, Massachusetts 02467, USA.
Pseudouridine (Ψ) is an abundant RNA chemical modification that can play critical roles in the biological functions of RNA, and RNA-therapeutic applications. Current Ψ detection methods are limited in identifying Ψs at base-resolution in U-rich sequence contexts, where Ψ occurs frequently. The N-cyclohexyl N'-(2-morpholinoethyl)carbodiimide (CMC) can selectively label Ψ in RNA by forming the CMC-Ψ adduct.
View Article and Find Full Text PDFContinuous Flow Oxidation of Alcohols Using TEMPO/NaOCl for the Selective and Scalable Synthesis of Aldehydes.
Org Process Res Dev
May 2024
School of Chemistry, University College Dublin, Science Centre South, Belfield D04 N2E5, Ireland.
A simple and benign continuous flow oxidation protocol for the selective conversion of primary and secondary alcohols into their respective aldehyde and ketone products is reported. This approach makes use of catalytic amounts of TEMPO in combination with sodium bromide and sodium hypochlorite in a biphasic solvent system. A variety of substrates are tolerated including those containing heterocycles based on potentially sensitive nitrogen and sulfur moieties.
View Article and Find Full Text PDFUnveiling the Untapped Potential of Bertagnini's Salts in Microwave-Assisted Synthesis of Quinazolinones.
Molecules
April 2024
Dipartimento di Scienze Chimiche e Geologiche, Università degli Studi di Cagliari, Cittadella Universitaria, 09042 Cagliari, Italy.
Microwave-assisted organic synthesis (MAOS) has emerged as a transformative technique in organic chemistry, significantly enhancing the speed, efficiency, and selectivity of chemical reactions. In our research, we have employed microwave irradiation to expedite the synthesis of quinazolinones, using water as an eco-friendly solvent and thereby adhering to the principles of green chemistry. Notably, the purification of the product was achieved without the need for column chromatography, thus streamlining the process.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!