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Inactivation Validation of Ebola, Marburg, and Lassa Viruses in AVL and Ethanol-Treated Viral Cultures.
Viruses
August 2024
National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB R3E 3R2, Canada.
High-consequence pathogens such as the Ebola, Marburg, and Lassa viruses are handled in maximum-containment biosafety level 4 (BSL-4) laboratories. Genetic material is often isolated from such viruses and subsequently removed from BSL-4 laboratories for a multitude of downstream analyses using readily accessible technologies and equipment available at lower-biosafety level laboratories. However, it is essential to ensure that these materials are free of viable viruses before removal from BSL-4 laboratories to guarantee sample safety.
View Article and Find Full Text PDFUV-Inactivated rVSV-M2e-Based Influenza Vaccine Protected against the H1N1 Influenza Challenge.
Front Biosci (Landmark Ed)
May 2024
Laboratory of Molecular Human Retrovirology, University of Manitoba, Winnipeg, MB R3T 2N2, Canada.
Article Synopsis
- The study explores the immune responses and protective capabilities of a UV-inactivated recombinant vesicular stomatitis virus (rVSV) vector that includes a fusion protein for influenza and a targeting domain for dendritic cells.
- The research demonstrated that both live and UV-inactivated rVSV-EΔM-tM2e induced strong immune responses against various influenza strains, showing effectiveness in protecting mice from H1N1 challenges.
- The findings suggest that UV-inactivated rVSV-EΔM-tM2e could be a viable candidate for developing an inactivated vaccine against multiple strains of influenza.
Preservation of scRNA-Seq Libraries Using Existing Inactivation Protocols.
Pathogens
February 2024
Florida Research and Innovation Center, Cleveland Clinic Lerner Research Institute, Port Saint Lucie, FL 34987, USA.
Single-cell RNA sequencing has soared in popularity in recent years. The ability to deeply profile the states of individual cells during the course of disease or infection has helped to expand our knowledge of coordinated responses. However, significant challenges arise when performing this analysis in high containment settings such as biosafety level 3 (BSL-3), BSL-3+ and BSL-4.
View Article and Find Full Text PDFPreparing for another Ebola Outbreak: The impact of viral inactivation methods on commonly measured biochemistry analytes in plasma and urine.
Clin Biochem
February 2024
Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada; Department of Pathology and Laboratory Medicine, Mount Sinai Hospital, Toronto, ON, Canada. Electronic address:
Introduction: Infectious specimens containing viruses like Ebola require sample manipulation to ensure the safety of laboratory staff, which may negatively impact biochemistry test results. We evaluated the impact of viral inactivation methods on 25 biochemistry analytes in plasma, and seven biochemistry analytes in urine.
Methods: Fifteen lithium heparinized plasma specimens with and without gel underwent the following viral inactivation methods: 1) untreated, 2) Triton X-100 treatment, 2) heated for 60 min then Triton X-100 treatment, 3) heated for 60 min, 4) heated for 75 min, and 5) heated for 90 min.
Unexpected thermal stability of two enveloped megaviruses, Emiliania huxleyi virus and African swine fever virus, as measured by viability PCR.
Virol J
January 2024
Department of Animal Science, University of Minnesota, St. Paul, MN, 55108, USA.
Background: The particle structure of Emiliania huxleyi virus (EhV), an algal infecting member of nucleocytoplasmic large DNA viruses (NCLDVs), contains an outer lipid membrane envelope similar to that found in animal viruses such as African swine fever virus (ASFV). Despite both being enveloped NCLDVs, EhV and ASFV are known for their stability outside their host environment.
Method: Here we report for the first time, the application of a viability qPCR (V-qPCR) method to describe the unprecedented and similar virion thermal stability of both EhV and ASFV.
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