The methods for the determination of Aconitum alkaloids in Radix Aconiti Kusnezoffii were established. Volumetric analysis with the indicator of methyl red-bromcresol green and colorimetric analysis with acid dye of bromecresol green under the wavelength of 416 nm were used for the assay of total alkaloids. Colorimetric analysis of hydroxamic acid-Fe was for the assay of total ester-diterpene type Aconitum alkaloids. Three kinds of diester-diterpene type Aconitum alkaloids (aconitine, hypaconitine and mesaconitine) were determined by HPLC method. The HPLC system consisted of C18 as the stationary phase and acetonitrile: buffer (acetic acid-triethylamine, pH 6.25) = 71:29 as the mobile phase. The wavelength was 235 nm.
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Heliyon
January 2025
Department of Cerebrovascular Disease, Hainan General Hospital, Hainan Affiliated Hospital of Hainan Medical University, Haikou, Hainan 570311, PR China.
Gliomas are the most common primary tumors of the nervous system, which is generally treated using adjuvant chemotherapy following surgical resection. However, patient survival time is still short, and there is currently no successful treatment for highly malignant gliomas. Bullatine A (BLA) is a diterpenoid alkaloid of the genus Aconitum which antirheumatic and anti-inflammatory pharmacological properties.
View Article and Find Full Text PDFAnticancer Agents Med Chem
January 2025
Pharmacy Department of Affiliated Traditional Chinese Medicine Hospital of Xinjiang Medical University, Urumqi, 830054, China.
Introduction/objective: The alkaloids of songorine, aconitine, and benzoylaconitine, as the processed products of Aconitum soongaricum Stapf., can significantly inhibit the migration and invasion of ovarian cancer cells in vitro. Herein, we studied the in vivo role and mechanism of these natural products in processed A.
View Article and Find Full Text PDFChem Biodivers
January 2025
Yunnan University, School of Chemical Science and Technologe, Cuihu Bei Road, Kunming, CHINA.
Kaurane-class diterpenoid alkaloids (DAs), an important group of C20-DAs, include mainly veatchine-, napelline-, anopterine- and tricalysiamide-type DAs and several types of DAs with novel skeletons discovered in recent years. To date, approximately 81 compounds belonging to this class of DAs have been isolated from plants in 5 families and 7 genera. Among them, Aconitum is the most important source of this class of DAs.
View Article and Find Full Text PDFInt J Mol Sci
January 2025
Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650500, China.
() is an important medicinal plant in the Aconitum genus that is known for its diterpenoid alkaloids, which exhibit significant pharmacological activity and toxicity, thus making it valuable for both medicinal use and as a biopesticide. Although the biosynthesis of terpenoids is well characterized, the potential gene regulatory role of microRNAs (miRNAs) in terpenoid biosynthesis in remains unclear, and further research is needed to explore this aspect in this species. In this study, miRNA sequencing was conducted to analyze the miRNA population and its targets in .
View Article and Find Full Text PDFMolecules
December 2024
State Key Laboratory of Southwestern Chinese Medicine Resources, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China.
stands out among the Ranunculaceae family for its notable use as an ornamental and medicinal plant. Diterpenoid alkaloids (DAs), the characteristic compounds of , have been found to have effective analgesic and anti-inflammatory effects. Despite their medicinal potential, the toxicity of most DAs restricts the direct use of in traditional medicine, necessitating complex processing before use.
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