AI Article Synopsis
- The study examines how the pseudorabies virus (PrV) affects apoptosis (cell death) in suckling piglets, focusing on the Ea strain of the virus.
- Various tissue samples were collected from infected piglets, and several methods like electron microscopy and TUNEL staining were used to identify apoptotic cells.
- It was found that the PrV infection primarily induced apoptosis in lymphoid tissues, weakening the immune system and leading to the death of piglets, while establishing a latent infection in nerve tissues by preventing apoptosis in those cells.
Article Abstract
To study induction or inhibition of apoptosis by pseudorabies virus (PrV) Ea strain in suckling piglets, Aujeszky s disease was replicated by artificially inoculating 15 day-old piglets with PrV-Ea strain. Various tissue sections, such as lymphoid tissues and nervous tissues were collected. Transmission electron microscopy, DNA fragmentation assay, and in situ terminal-deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) staining were carried to analyze apoptotic cells. It was shown that piglets infected with PrV-Ea strain took on typical clinical symptoms and apoptotic cells were found in lymphoid tissues but not in nerve tissues. The results indicated that the PrV infection caused apoptosis in a big number of lymphatic cells, thus leading to death of suckling piglets due to lowered immune function. It might play an important role in PrV s attack on immune system. A latent infection of pseudorabies virus was established in the neuronal tissue cells by inhibition of nervous cell apoptosis; this might be the way for PrV to establish latent infection, leading to sporadic recurrence of the disease.
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