A small amount of fluid is usually present in the space between the posterior surface of the silicone oil bubble and the retinal surface in silicone oil-filled eyes. A new technique is described for sampling this fluid using a 27-gauge cannula mounted on an empty tuberculin syringe that is connected to a 10-mL syringe through tubing.
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Hereditas
January 2025
Key Laboratory of Reproductive Health Diseases Research and Translation of Ministry of Education & Key Laboratory of Human Reproductive Medicine and Genetic Research of Hainan Provincie & Hainan Provincial Clinical Research Center for Thalassemia, The First Affiliated Hospital of Hainan Medical University, Hainan Medical University, Haikou, Hainan, 571101, China.
Background: The dynein cytoplasmic two heavy chain 1 (DYNC2H1) gene encodes a cytoplasmic dynein subunit. Cytoplasmic dyneins transport cargo towards the minus end of microtubules and are thus termed the "retrograde" cellular motor. Mutations in DYNC2H1 are the main causative mutations of short rib-thoracic dysplasia syndrome type III with or without polydactyly (SRTD3).
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January 2025
Lunar and Planetary Laboratory, University of Arizona, Tucson, AZ, USA.
Evaporation or freezing of water-rich fluids with dilute concentrations of dissolved salts can produce brines, as observed in closed basins on Earth and detected by remote sensing on icy bodies in the outer Solar System. The mineralogical evolution of these brines is well understood in regard to terrestrial environments, but poorly constrained for extraterrestrial systems owing to a lack of direct sampling. Here we report the occurrence of salt minerals in samples of the asteroid (101955) Bennu returned by the OSIRIS-REx mission.
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January 2025
Fujian Key Laboratory of Molecular Neurology, Department of Neurology, Institute of Neurology of First Affiliated Hospital, Institute of Neuroscience, Fujian Medical University, Fuzhou, 350005, China.
The application of metagenomic next-generation sequencing (mNGS) in the diagnosis of cryptococcal meningitis is relatively under characterized. Here, we retrospectively evaluated data from cryptococcal meningitis patients who were tested using mNGS and/or routine testing, including fungal culture, India ink staining, and cryptococcal antigen (CrAg) testing. The performance of mNGS was then assessed.
View Article and Find Full Text PDFAnal Chim Acta
March 2025
Holosensor Medical Technology Ltd, Room 12, No. 1798, Zhonghuayuan West Road, Yushan Town, Suzhou, 215000, China; Department of Veterinary Medicine, University of Cambridge, Cambridge, UK. Electronic address:
Rapid and sensitive protein detection methods are of benefit to clinical diagnosis, pathological mechanism research, and infection prevention. However, routine protein detection technologies, such as enzyme-linked immunosorbent assay and Western blot, suffer from low sensitivity, poor quantification and labourious operation. Herein, we developed a fully automated protein analysis system to conduct fast protein quantification at the single molecular level.
View Article and Find Full Text PDFTalanta
January 2025
International Ph. D Program in Innovative Technology of Biomedical Engineering & Medical Devices, Ming Chi University of Technology, New Taipei City 243303, Taiwan; Research Center for Intelligence Medical Devices, Ming Chi University of Technology, New Taipei City 243303, Taiwan. Electronic address:
Nanocomposites that mimic the characteristics of enzymes, commonly as nanozymes, can function as an efficient sensing material with high selectivity towards the targeted biological macromolecule. These nanozymes overcome of the challenges that arise when using natural enzymes as sensing material. This study presents a novel nanozyme, Copper Selenite (CuSeO) nanoparticles mounted on f-CNF, to electrochemically determine a potential cardiovascular biomarker, Glutathione (GSH).
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