The design and synthesis of a biotin-tagged photoreactive analogue C-4 of the cholesterol absorption inhibitor Ezetimibe is described. Photoaffinity labeling of intestinal brush border membrane vesicles with C-4 and subsequent streptavidin-biotin chromatography leads to selective extraction of a 145 kDa integral membrane protein as the molecular target for cholesterol absorption inhibitors.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s0968-0896(03)00047-6 | DOI Listing |
Publication Analysis
Top Keywords
cholesterol absorption
12
synthesis biotin-tagged
8
absorption inhibitors
8
biotin-tagged photoaffinity
4
photoaffinity probe
4
probe 2-azetidinone
4
2-azetidinone cholesterol
4
inhibitors design
4
design synthesis
4
biotin-tagged photoreactive
4
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!