The development of thrombin inhibitors could provide invaluable progress for antithrombotic therapy. In this paper, we report the cloning, purification and biochemical characterization of dipetarudin, a chimeric thrombin inhibitor composed of the N-terminal head structure of dipetalogastin II, the strongest inhibitor from the assassin bug Dipetalogaster maximus, and the exosite 1 blocking segment of hirudin, connected through a five glycine linker. The cloning of dipetarudin was performed by a simple method which had not been used previously to clone chimeras. Biochemical characterization of dipetarudin revealed that it is a slow, tight-binding inhibitor with a molecular mass (M(r)=7560) and a thrombin inhibitory activity (K(i)=446 fM) comparable to r-hirudin.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/s1570-0232(02)00739-0 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Evaluation of the importance of sediment organic matter composition for CH production by microcosm tests with and without addition of natural sources (cyanobacterial biomass and riparian pasture) in two subtropical, eutrophic reservoirs.
Environ Sci Pollut Res Int
January 2025
Facultad de Ciencias, Sección Limnología, IECA, Universidad de la República, Montevideo, Uruguay.
The biochemical composition of sediments, which depends on the origin of the organic matter (OM), is decisive in methane (CH) production. This study aimed to determine the CH produced under anaerobic conditions from different substrates: native reservoir sediments and sediments with the addition of complex OM from Microcystis spp. blooms and terrestrial plants (pasture), alongside the biochemical characterization of the substrates used.
View Article and Find Full Text PDFProtein biochemistry and engineering drive the development of a carbonic anhydrase-based carbon dioxide sequestration strategy.
FEBS J
January 2025
'The Protein Factory 2.0', Dipartimento di Biotecnologie e Scienze della Vita, Università degli Studi dell'Insubria, Varese, Italy.
The sequestration of carbon dioxide using carbonic anhydrase (CA) is one of the most effective methods for mitigating global warming. The burning of fossil fuels releases large quantities of flue gas; because of its high temperature and of the alkaline conditions required for CaCO precipitation in the mineralization process, thermo-alkali-stable CAs are needed. In this context, Manyumwa et al.
View Article and Find Full Text PDFEffect of glutathione reductase on photosystem II characterization and reactive oxygen species metabolism in cotton cytoplasmic male sterile line Jin A.
Plant J
January 2025
College of Agriculture, Shanxi Agricultural University, Taigu, Shanxi, 030801, China.
Glutathione reductase (GR) maintains the cellular redox state by reducing oxidized glutathione to glutathione (GSH), which regulates antioxidant defense. Additionally, GR plays an essential role in photosynthesis; however, the mechanism by which GR regulates photosystem II (PSII) is largely unknown. We identified six, three, and three GR genes in Gossypium hirsutum, Gossypium arboreum, and Gossypium raimondii, respectively.
View Article and Find Full Text PDFCase report: Intradural-extramedullary cervical spine clear cell meningioma mimicking a schwannoma in a child.
Front Oncol
January 2025
Department of Neurosurgery, West China Hospital, Sichuan University, Chengdu, Sichuan, China.
Clear cell meningioma (CCM) is an exceedingly rare subtype of meningioma, with spinal occurrences being even more uncommon. It predominantly affects children and is characterized by a high recurrence rate and poor prognosis, posing significant challenges for clinical treatment. Currently, gross total resection (GTR) is the best approach to reduce recurrence and improve prognosis in these patients.
View Article and Find Full Text PDFProtein phosphatases are critical for regulating cell signaling, cell cycle, and cell fate decisions, and their dysregulation leads to an array of human diseases like cancer. The dual specificity phosphatases (DUSPs) have emerged as important factors driving tumorigenesis and cancer therapy resistance. DUSP12 is a poorly characterized atypical DUSP widely conserved throughout evolution.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!