AI Article Synopsis
- Leptin, a hormone produced by fat cells, regulates food intake and energy use through its interaction with specific receptors.
- Vanadate, a potent inhibitor of certain enzymes, was found to enhance the phosphorylation of key signaling proteins (JAK2 and STAT3) in cells responding to leptin, potentially boosting leptin's effects.
- These findings suggest that PTP inhibitors like vanadate could be useful in developing treatments for obesity by enhancing leptin signaling.
Article Abstract
Leptin, the product of the ob gene, is an adipocyte-derived hormone that plays a key role in the control of food intake and energy expenditure. Leptin acts through receptors that belong to a member of the class I cytokine receptor family. It has been demonstrated that the SH2 domain-containing tyrosine phosphatase 2 (SHP-2) negatively regulates STAT3-mediated transcriptional activation through long form leptin receptor (OBRb). Vanadate has been shown to be a potent and selective inhibitor of PTPase activity in vitro. In this study, we have demonstrated that vanadate increases leptin-induced JAK2 and STAT3 phosphorylation in CHO cells expressing OBRb. The increased leptin-dependent luciferase activity of SOCS3 gene was also seen in vanadate-treated cell. Furthermore, vanadate reversed the inhibitory effects of SOCS3 on leptin-induced STAT3 phosphorylation. The present findings suggest that PTP inhibitors including vanadate and vanadate-derived compounds could be used as a therapeutic agent in the treatment of obesity.
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| http://dx.doi.org/10.1016/s0006-291x(03)00264-x | DOI Listing |
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