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Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles. | LitMetric

Artificial viruses and their application to gene delivery. Size-controlled gene coating with glycocluster nanoparticles.

J Am Chem Soc

Department of Synthetic Chemistry and Biological Chemistry, Graduate School of Engineering, Kyoto University, Yoshida, Sakyo-ku, Kyoto 606-8501, Japan.

Published: March 2003

AI Article Synopsis

  • * The design features approximately 2 GNPs for every 10 base pairs of DNA, creating compact structures that are well-shielded from charges and can deliver genes into cell cultures without harmful effects.
  • * These "glycoviruses" provide a novel method for gene delivery, moving away from traditional viral and cationic vectors, and highlight the potential of saccharide clusters for building nanoscale structures.

Article Abstract

Number- and size-controlled macromolecular associations are common in biology with viruses as a typical example. We report here a novel example of artificial viruses, in which the double-helical DNA is coated with 4-nm sized neutral glycocluster nanoparticles (GNPs) with a coating stoichiometry of approximately 2 GNPs per helical pitch (10 base pairs), where GNP arises from micellization of a cone-shaped, quadruple-chain glycocluster amphiphile having eight saccharide moieties with beta-glucoside termini on the calix[4]resorcarene macrocycle. The resulting "glycoviruses" are compactly packed (54 nm in the case of 7040 base-pair plasmid pCMVluc), are well charge-shielded (zeta congruent with approximately 0 mV), and effectively transfect cell cultures without notable cytotoxicity. The use of artificial viral vectors thus allows a new (nonamine/noncationic/nonpolymeric) access to gene delivery, a potential but still tough subject which has been studied extensively over the last 15 years by using viral or amine-based cationic vectors. The remarkable adhesion-manipulation ability of saccharide clusters also provides a strategy of bottom-up construction of nanometric or mesoscopic sizes.

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Source
http://dx.doi.org/10.1021/ja029608tDOI Listing

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