AI Article Synopsis
- The study aimed to investigate the mRNA expression of TGFbetas, TbetaRII, and telomerase activity in rat liver during cholangiocarcinogenesis, induced by a specific chemical.
- In situ hybridization and telomerase assays were employed to analyze tissue samples, revealing increased expression of TGFbeta1 and TbetaRII in various affected cholangiocytes and positive telomerase activity in those cells.
- The findings suggest that TGFbetas, TbetaRII, and telomerase activity play significant roles in the progression of cholangiocarcinoma and the formation of the surrounding tissue stroma.
Article Abstract
Aim: Initial report on the in situ examination of the mRNA expression of transforming growth factor betas (TGFbetas), TGFbeta type II receptor (TbetaRII) and telomerase activity in the experimental rat liver tissue during cholangiocarcinogenesis.
Methods: Rat liver cholangiocarcinogenesis was induced by 3'-methyl 4-dimethylazobenzene (3'Me-DAB). In situ hybridization was used to examine the TGFbetas) and TGFbeta type II receptor (TbetaRII) mRNA, in situ TRAP was used to check the telomerase activity in the tissue samples.
Results: There was no TGFbetas, TbetaRII mRNA expression or telomerase activity in the control rat cholangiocytes. The expression of TGFbeta1, TbetaRII was increased in regenerative, hyperplastic, dysplastic cholangiocytes and cholangiocarcinoma (CC) cells. The expression of TGFbeta2 mRNA was observed in only a part of hyperplastic, dysplastic cholangiocytes. TGFbeta3 expression was very weak, only in hyperplastic lesion. There was positive telomerase activity in the regenerative, hyperplastic, dysplastic cholangiocytes, and CC cells. Stroma fibroblasts of these lesions also showed positive TGFbetas, TbetaRII mRNA expression and telomerase activity.
Conclusion: There were TGFbetas, TbetaRII expression and telomerase activity in hyperplastic, dysplastic cholangiocytes, cholangiocarcinoma cells as well as in stroma fibroblasts during cholangiocarcinogenesis. Their expression or activity is important in cholangiocarcinogenesis andstroma formation.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4621588 | PMC |
| http://dx.doi.org/10.3748/wjg.v9.i3.590 | DOI Listing |
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