Combined therapy of allantoin, metronidazole, dexamethasone on the prevention of intra-abdominal adhesion in dogs and its quantitative analysis.

Aim: To observe the preventive effects of combined therapy of AMD (allantoin, metronidazolem and dexamethasone in combination) on intra-abdominal adhesion in dogs.

Methods: 20 dogs of both sexes were used in this study. After laparotomy under anesthesia, 2 cm section of cecal end was clamped and ligated, then 1 cm cecum section was cut and another 1 cm was kept. The cecum stump was closed with purse-string suture. Both parietal and visceral peritonea were stripped for an area of about 3X4 cm(2). Before the skin closure, the animals were divided into two groups randomly. The abdominal cavities in Group AMD (n=10) were rinsed by 200 ml of AMD solution, and with 50 ml left, whereas the control (n=10) received the equal volume of normal saline. After 7 d, the degree of intra-abdominal adhesions was evaluated by using the score method of ultrasonography and traditional dissection.

Results: Compared with the control, both the ultrasonography and traditional dissection scores in Group AMD were significantly decreased that marked as 2.0+/-1.25 vs 3.3+/-0.82 and 1.91+/-0.83 vs 3.3+/-0.82 respectively (P<0.01).

Conclusion: The combined therapy of AMD is an effective way to prevent intra-abdominal adhesion, and ultrasonography is an useful tool to diagnose intra-abdominal adhesion.