The GATA factor Pannier activates proneural achaete/scute (ac/sc) expression during development of the sensory organs of Drosophila through enhancer binding. Chip bridges Pannier with the (Ac/Sc)-Daughterless heterodimers bound to the promoter and facilitates the enhancer-promoter communication required for proneural development. We show here that this communication is regulated by Osa, which is recruited by Pannier and Chip. Osa belongs to Brahma chromatin remodeling complexes and we show that Osa negatively regulates ac/sc. Consequently, Pannier and Chip also play an essential role during repression of proneural gene expression. Our study suggests that altering chromatin structure is essential for regulation of enhancer-promoter communication.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC196006 | PMC |
| http://dx.doi.org/10.1101/gad.255703 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Geometric variations in nucleosomal DNA dictate higher-order chromatin structure and enhancer-promoter communication.
J Chem Phys
December 2024
Department of Chemistry and Chemical Biology, Center for Quantitative Biology, Rutgers, The State University of New Jersey, 610 Taylor Road, Piscataway, New Jersey 08854, USA.
The dynamic organization of chromatin plays an essential role in the regulation of genetic activity, interconverting between open and compact forms at the global level. The mechanisms underlying these large-scale changes remain a topic of widespread interest. The simulations of nucleosome-decorated DNA reported herein reveal profound effects of the nucleosome itself on overall chromatin properties.
View Article and Find Full Text PDFTranscription-coupled changes in genomic region proximities during transcriptional bursting.
Sci Adv
December 2024
Division of Gene Expression Dynamics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan.
The orchestration of our genes heavily relies on coordinated communication between enhancers and promoters, yet the mechanisms behind this dynamic interplay during active transcription remain unclear. Here, we investigated enhancer-promoter (E-P) interactions in relation to transcriptional bursting in mouse embryonic stem cells using sequential DNA/RNA/immunofluorescence-fluorescence in situ hybridization analyses. Our data reveal that the active state of specific genes is characterized by specific proximities between different genomic regions and the accumulation of transcriptional regulatory factors.
View Article and Find Full Text PDFMultiple allelic configurations govern long-range Shh enhancer-promoter communication in the embryonic forebrain.
Mol Cell
December 2024
Department of Genetics, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Penn Epigenetics Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA. Electronic address:
Article Synopsis
- Developmental gene regulation relies on long-range communication between enhancers and promoters, which is not fully understood without considering the surrounding chromatin.
- Researchers studied the Sonic hedgehog (Shh) regulatory region in mouse brains to understand its structural configurations, discovering that it often maintains a compact form that can adopt various layouts regardless of Shh expression.
- They found that long-range enhancer-promoter interactions occur through both dependent and independent mechanisms of Shh expression, involving specific binding sites and active enhancers, leading to a proposed model that coordinates gene expression patterns.
Genetic factors mediating long-range enhancer-promoter communication in mammalian development.
Curr Opin Genet Dev
November 2024
Department of Developmental and Cell Biology, University of California, Irvine, CA 92967, USA. Electronic address:
Article Synopsis
- * Short-range enhancers operate by connecting with transcription factors and other proteins when they are close to gene promoters, while medium- and long-range enhancers use additional mechanisms to interact over greater distances.
- * Understanding how these enhancers work, especially their interactions over large genomic areas, is important for gene activation in mammals and will require further research to explore their role across different species and cell types.
Systematic screening of enhancer-blocking insulators in Drosophila identifies their DNA sequence determinants.
Dev Cell
November 2024
Center for Integrative Genomics, University of Lausanne, 1015 Lausanne, Switzerland. Electronic address:
Long-range transcriptional activation of gene promoters by abundant enhancers in animal genomes calls for mechanisms to limit inappropriate regulation. DNA elements called insulators serve this purpose by shielding promoters from an enhancer when interposed. Unlike promoters and enhancers, insulators have not been systematically characterized due to lacking high-throughput screening assays, and questions regarding how insulators are distributed and encoded in the genome remain.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!